細胞機能変換技術研究チーム

再生医療には様々な有用細胞を得る必要がありますが、iPS細胞のように受精卵に近い状態に戻してから再分化させるのみならず、分化細胞から直接変換することも可能です。こういった細胞機能変換を目指して、細胞の転写状態、転写制御ネットワーク、エピゲノム、ノンコーディングRNAの知識を統合して、ゲノム領域特異的なエピゲノム修飾変換法を含む新たな技術開発を行います。こうした技術は幹細胞の分化や安全性の研究や、疾患治療にも応用が期待されます。

研究分野

生物学 & 生化学 / 分子生物 & 遺伝学

研究テーマ

• 細胞機能変換技術の開発
• 哺乳動物における転写制御ネットワーク解析
• 薬物作用のプロモーター解析

主要論文

1. H. Suzuki, A. R. Forrest, E. van Nimwegen et al. "The transcriptional network that controls growth arrest , differentiation in a human myeloid leukemia cell line", Nat Genet, 41, 553-562 (2009)
2. Ravasi T, Suzuki H, Cannistraci CV, Katayama S, Bajic VB et al. "An atlas of combinatorial transcriptional regulation in mouse and man", Cell. 2010 Mar 5;140(5):744-52.
3. Suzuki T, Nakano-Ikegaya M, Yabukami-Okuda H, de Hoon M, Severin J, Saga-Hatano S, Shin J, Kubosaki A, Simon C, Hasegawa Y, Hayashizaki Y, Suzuki H. "Reconstruction of Monocyte Transcriptional Regulatory Network Accompanies Monocytic Functions in Human Fibroblasts.", PLoS One. 2012, 7(3), e33474.
4. Shin JW, Suzuki T, Ninomiya N, Kishima M, Hasegawa Y, Kubosaki A, Yabukami H, Hayashizaki Y, Suzuki H. "Establishment of single-cell screening system for the rapid identification of transcriptional modulators involved in direct cell reprogramming.", Nucleic Acids Res. 40(21):e165 (2012).
5. Hasegawa Y, Takahashi N, Forrest AR, Shin JW, Kinoshita Y, Suzuki H, Hayashizaki Y. "CC chemokine ligand 2 and leukemia inhibitory factor cooperatively promote pluripotency in mouse induced pluripotent cells." Stem Cells. 2011 Aug;29 (8): 1196-205.
6. Schroder K, Irvine KM, Taylor MS, Bokil NJ, Le Cao KA, Masterman KA, Labzin LI, Semple CA, Kapetanovic R, Fairbairn L, Akalin A, Faulkner GJ, Baillie JK, Gongora M, Daub CO, Kawaji H, McLachlan GJ, Goldman N, Grimmond SM, Carninci P, Suzuki H, Hayashizaki Y, Lenhard B, Hume DA, Sweet MJ. "Conservation and divergence in Toll-like receptor 4-regulated gene expression in primary human versus mouse macrophages." Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):E944-53.
7. Vitezic M, Lassmann T, Forrest AR, Suzuki M, Tomaru Y, Kawai J, Carninci P, Suzuki H, Hayashizaki Y, Daub CO. "Building promoter aware transcriptional regulatory networks using siRNA perturbation" , deepCAGE. Nucleic Acids Res. 2010 Dec 1;38(22):8141-8.
8. Forrest AR, Kanamori-Katayama M, Tomaru Y, Lassmann T, Ninomiya N, Takahashi Y, de Hoon MJ, Kubosaki A, Kaiho A, Suzuki M, Yasuda J, Kawai J, Hayashizaki Y, Hume DA, Suzuki H. "Induction of microRNAs, mir-155, mir-222, mir-424 , mir-503, promotes monocytic differentiation through combinatorial regulation." Leukemia. 2010 Feb;24(2):460-6. Epub 2009 Dec 3.
9. C. Ogawa, K. Usui, F. Ito, M. Itoh, Y. Hayashizaki, , H. Suzuki, "Role of survival motor neuron complex components in small nuclear ribonucleoprotein assembly", J Biol Chem, 284,14609-17 (2009)
10. K. Usui, T. Maki, F. Ito, A. Suenaga, S. Kidoaki, M. Itoh, M. Taiji, T. Matsuda, Y. Hayashizaki, and H. Suzuki, "Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks", Protein Sci, 18, 960-969 (2009)