変異マウス開発ユニット

今日の生物学・医学研究は、どれぐらい効率よく変異マウスが作成でき、取り扱えるかに左右されているといっても過言ではありません。当研究チームは我が国における発生・再生研究発展のため、CDB のみならず国内外の研究者と共同して、様々な遺伝子について変異マウスを作成・開発しています。研究者からの遺伝子情報をもとに、ベクターの構築、ES 細胞の培養、相同組み換え体の単離、キメラマウス・トランスジェニックマウスの作出は当研究チームがすべて行います。マウス胚でのイメージングのための各種マウスの作成と胚培養、顕微技術の開発に力点をおいています。

研究テーマ

• 発生・再生研究に有用な変異マウスの開発
• マウス胚でのイメージング技術の開発
• 遺伝的及び顕微的マウス胚操作法の開発

主要論文

1. Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T.:
   "Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter."
   Development. 140(1):237-246, 2013
2. Shinohara R, Thumkeo D, Kamijo H, Kaneko N, Sawamoto K, Watanabe K, Takebayashi H, Kiyonari H, Ishizaki T, Furuyashiki T, Narumiya S.:
   "A role for mDia, a Rho-regulated actin nucleator, in tangential migration of interneuron precursors."
   Nature Neuroscience. 15: 373-380, 2012
3. Fukuhara S, Simmons S, Kawamura S, Inoue A, Orba Y, Tokudome T, Sunden Y, Arai Y, Moriwaki K, Ishida J, Uemura A, Kiyonari H, Abe T, Fukamizu A, Hirashima M, Sawa H, Aoki J, Ishii M, Mochizuki N.:
   "The sphingosine-1-phosphate transporter Spns2 expressed on endothelial cells regulates lymphocyte trafficking in mice."
   The Journal of Clinical Investigation.122(4), 1416-1426, 2012
4. Okuda-Ashitaka E, Minami T, Tsubouchi S, Kiyonari H, Iwamatsu A, Noda T, Hanada H and Ito S.:
   "Identification of NIPSNAP1 as a nocistatin-interacting protein involving pain transmission."
   J. Biol. Chem. 287(13), 10403-10413, 2012
5. Aoyama K, Wang F, Matsumura N, Kiyonari H, Shioi G, Tanaka K, Kinoshita C, Kikuchi-Utsumi K, Watabe M, Nakaki T.:
   "Increased neuronal glutathione and neuroprotection in GTRAP3-18-deficient mice."
   Neurobiol Dis. 45(3), 973-982, 2012
6. Niisato E, Nagai J, Yamashita N, Abe T, Kiyonari H, Goshima Y and Ohshia T.:
   "CRMP4 suppresses apical dendrite bifurcation of CA1 pyramidal neurons in the mouse hippocampus."
   Developmental Neurobiology. 72(11), 1447-1457, 2012
7. Tao H, Inoue K, Kiyonari H, Bassuk AG, Axelrod JD, Sasaki H, Aizawa S, Ueno N.:
   "Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis."
   Dev Biol. 364(2), 138-148, 2012
8. Kanemaru K, Nakamura Y, Sato K, Kojima R, Takahashi S, Yamaguchi M, Ichinohe M, Kiyonari H, Shioi G, Kabashima K, Nakahigashi K, Asagiri M, Jamora C, Yamaguchi H, Fukami K.:
   "Epidermal phospholipase Cδ1 regulates granulocyte counts and systemic interleukin-17 levels in mice."
   Nat Commun. 2012 jul 17;3:393
9. Nishimur R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G, Yamaguchi A, Tsumaki N, Akiyama H, Yoneda T.:
   "Osterix Regulates Calcification and Degradation of Chondrogenic Matrices Through Matrix Metalloproteinase(MMP13) Expression in Association with Transcription Factor Runx2 During Endochondral Ossification."
   J Biol Chem. 287(40), 33179-33190, 2012
10. Inazuka F, Sugiyama N, Tomita M, Abe T, Shioi G, Esumi H.:
    "Muscle-specific knock-out of NUAK family SNF1-like kinase 1(NUAK1)prevents high fat diet-induced glucose intolerance."
    J Biol Chem. 287(20), 16379-16389, 2012