生体情報統合技術開発チーム

• 免疫機構（自己免疫疾患の発症および炎症の制御）におけるNF-kB/RelAの制御システム
• 骨代謝機構におけるNF-kB/RelAの関与
• 造血幹細胞増殖機構のNF-kB/RelA制御システム
• 造血幹細胞を体外で増幅するための技術開発
• ES細胞やiPS細胞などの多能性幹細胞の安全かつ効率のよい樹立法および安定した増幅法の開発
• 細胞に遺伝子を導入するためのウイルスベクターの開発と幹細胞生物学への応用

研究分野

生物学 & 生化学 / 分子生物 & 遺伝学 / 免疫学 / 臨床医学 / 植物 & 動物学

研究テーマ

• 造血幹細胞増殖機構の制御
• 免疫機構におけるNF-kB/RelAの制御
• 遺伝子導入用レンチウイルスの開発と幹細胞生物学への応用

主要論文

1. Nakano H, Doi T et al.
   Oxidative stress-dependent production of Interleukin 11 ameliorates drug-induced murine hepatitis through compensatory proliferation.
   Journal of Clinical Investigation (2011)
2. Maruyama T, Fukushima H, Nakao K, Shin M, Yasudsa H, Weih F, Doi T, Aoki K, Ohya K, Hsokawa R and Jimi E
   Processing of the NF-kB2 precursor, p100, to p52 is critical for RANKL-induced osteoclast differentiation
   Journal of Bone and Mineral Research (2009)
3. Yamazaki M, Fukushima H, Shin M, Katagiri T, Doi T, Takahashi T and Jimi E
   A proximal kappaB site in the IL-23 p19 promoter is responsible for RelA- and c-Rel-dependent transcription
   Journal of Biological Chemistry, 284; 35987-95 (2009)
4. Mise-Omata S, Kuroda E, Sugiura T, Yamashita U, Obata Y, and Doi TS
   The NF-kB RelA subunit confers resistance to Leishmania major by inducing NOS2 and Fas expression but not Th1 differentiation
   J. Immunol. 182;4910-4916 (2009)
5. Mise-Omata S, Kuroda E, Niikura J, Yamashita U, Obata Y, and Doi TS
   A proximal kappaB site in the IL-23 p19 promoter is responsible for RelA- and c-Rel-dependent transcription
   J. Immunol. 179, 6596-6603 (2007)
6. Inoue, S., Noda, S., Kashima, K., Nakada, K., Hayashi, J., and Miyoshi, H.
   Mitochondrial respiration defects modulate differentiation but not proliferation of hematopoietic stem and progenitor cells.
   FEBS Lett. 584, 3402-3409 (2010)
7. Noda, S., Ichikawa, H., and Miyoshi, H.
   Hematopoietic stem cell aging is associated with functional decline and delayed cell cycle progression.
   Biochem. Biophys. Res. Commun. 383, 210-215 (2009)
8. Shimizu, N., Noda, S., Katayama, K., Ichikawa, H., Kodama, H., and Miyoshi, H.
   Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6.
   Int. J. Hematol. 87, 239-245 (2008)
9. Noda, S., Horiguchi, K., Ichikawa, H., and Miyoshi, H.
   Repopulating activity of ex vivo-expanded murine hematopoietic stem cells resides in the CD48-c-Kit+Sca-1+lineage marker- cell population.
   Stem Cells 26, 646-655 (2008)
10. Inoue, S., Yokota, M., Nakada, K., Miyoshi, H., and Hayashi, J.
    Pathogenic mitochondrial DNA-induced respiration defects in hematopoietic cells result in anemia by suppressing erythroid differentiation.
    FEBS Lett. 581, 1910-1916 (2007)