遺伝工学基盤技術室

バイオリソース事業の本格実施にともない、リソースをより高度なクオリティにて維持・供給することが求められており、そのために必要な遺伝関連技術開発を行います。特に、確立が望まれている基盤的技術である核移植法、顕微授精法、各種幹細胞の樹立法ならびに胚・配偶子等リソース凍結法の開発を推進します。

研究テーマ

• 胚・配偶子凍結技術
• 顕微授精技術
• 核移植クローン技術
• 新規幹細胞開発技術

主要論文

1. Matoba S, Inoue K, Kohda K, Sugimioto M, Mizutani E, Ogonuki N, Nakamura T, Abe K, Nakano T, Ishino F, and Ogura A.:
   "RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos. "
   Proc Natl Acad Sci USA, 108, 20621-20626 (2011)
2. Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A.:
   "Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer."
   Science, 330, 496-499 (2010)
3. Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A.:
   "Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells."
   Proc Natl Acad Sci USA, 104, 12389-12394 (2007)
4. Ogonuki, N., Mochida, K., Miki, H., Inoue, K., Fray, M., Iwaki, T., Moriwaki, K., Obata, Y., Morozumi, K., Yanagimachi, R., and Ogura A.:
   "Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring"
   Proc. Natl. Acad. Sci. USA, 103, 13098-13103 (2006)
5. Inoue, K., Ogonuki, N., Miki, H., Hirose, M., Noda, S., Kim, J-M., Aoki, F., Miyoshi, H,, and Ogura A.:
   "Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer"
   J. Cell Sci. 119, 1985-1991(2006)
6. Inoue, K., Wakao, H., Ogonuki, N., Miki, H., Seino, K., Nambu-Wakao, R., Noda, S., Miyoshi, H., Koseki, H., Taniguchi, M., and Ogura, A.:
   "Generation of cloned mice by direct nuclear transfer from natural killer T cells"
   Curr. Biol. 15, 1114-1118 (2005)
7. Kanatsu-Shinohara, M., Inoue, K., Lee, J., Yoshimoto, M., Ogonuki, N., Miki, H., Baba, S., Kato, T., Kazuki, Y., Toyokuni, S., Oshimura, M., Heike, T., Nakahata, T., Ishino, F., Ogura, A., and Shinohara, T.:
   "Generation of pluripotent stem cells from neonatal mouse testis"
   Cell 119, 1001-1012 (2004)
8. Fulka, J Jr., Miyashita, N., Nagai, T., and Ogura, A.:
   "Do cloned mammals skip a reprogramming step"
   Nat. Biotechnol. 22, 25-26 (2004)
9. Ogonuki, N., Inoue, K., Yamamoto, Y., Noguchi, Y., Tanemura, K., Suzuki, O., Nakayama, H., Doi, K., Ohtomo, K., Satoh, M., Nishida, A., and Ogura, A.:
   "Early death of mice cloned from somatic cells"
   Nat. Genet. 30, 253-254 (2002)
10. Inoue, K., Kohda, T., Lee, J., Ogonuki, N., Mochida, K., Noguchi, Y., Tanemura, K., Kaneko-Ishino, T., Ishino, F., and Ogura, A.:
    "Faithful expression of imprinted genes in cloned mice"
    Science 295, 297 (2002)