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Genome Science Laboratory
Yoshihide HAYASHIZAKI
Chief Scientist
Yoshihide HAYASHIZAKI (M.D., Ph.D.)
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Research Areas

The Genome Science Laboratory has been developing basic technologies for functional genomics and exploring the genes resources. Our main activity, the establishment of the mouse full-length cDNA encyclopedia, has been carried over by Genome Exploration Research Group, Genomic Sciences Center which started in October, 1998. Our laboratory is now working on the development of new full-length cDNA library construction technologies to collect even more rarely expressed genes. Moreover, we are focusing on the analysis of alternatively splices mRNA, which do produce various functional proteins, and constructing the so-called super long cDNA libraries ( a good part of disease-related genes produce very long mRNAs, and therefore such kind of libraries are necessary), and have developed the CAGE(Cap Analysis Gene Expression) technology which allows collecting transcriptional starting points. The development of these key technologies has been contributing to discover disease-related genes, pharmaceutically relevant target genes and other commercially valuable targets. We have established a collaboration networks with many institutes and universities in Japan and abroad, and have used our originally developed technologies to obtain biological insights from disease models. With the Genome Network Project, in collaboration with the Genome Exploration Research Group, we are applying the high throughput system of CAGE to comprehensively collect transcriptome starting sites in order to map gene expression regulatory elements. These data constitute to be a basic platform to analyze the Genome Network.

Research Subject

  1. Development of technology for full-length cDNA library construction
  2. Analysis of alternative splicing
  3. Development of technologies for functional gene analysis
  4. Development of NanoLEGO

Related links

  1. RIKEN Advanced Science Institute Website_Laboratories PageNew Window
  2. RIKEN Omics Science Center WebsiteNew Window

List of Selected Publications

  1. Mitani, Y., Lezhava, A., Kawai, Y., Kikuchi, T., Oguchi-Katayama, A., Kogo, Y., Itoh, M., Miyagi, T., Takakura, H., Hoshi, K., Kato, C., Arakawa, T., Shibata, K., Fukui, K., Masui, R., Kuramitsu, S., Kiyotani, K., Chalk, A., Tsunekawa, K., Murakami, M., Kamataki, T., Oka, T., Shimada, H., Cizdziel, PE., and Hayashizaki, Y.:
    "Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology."
    Nat Methods. 4(3), 257-262 (2007).
  2. Carninci, P., Sandelin, A., Lenhard, B., Katayama, S., Shimokawa, K., Ponjavic, J., Semple, CA., Taylor, MS., Engstrom, PG,, Frith, MC., Forrest, AR., Alkema, WB., Tan, SL., Plessy, C., Kodzius, R., Ravasi, T., Kasukawa, T., Fukuda, S., Kanamori-Katayama, M., Kitazume, Y., Kawaji, H., Kai, C., Nakamura, M., Konno, H., Nakano, K., Mottagui-Tabar, S., Arner, P., Chesi, A., Gustincich, S., Persichetti, F., Suzuki, H., Grimmond, SM., Wells, CA., Orlando, V., Wahlestedt, C., Liu, ET., Harbers, M., Kawai, J., Bajic, VB., Hume, DA., and Hayashizaki, Y.:
    "Genome-wide analysis of mammalian promoter architecture and evolution."
    Nat Genet, 38, 626-635 (2006).
  3. The FANTOM Consortium, RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group):
    "The Transcriptional Landscape of the Mammalian Genome."
    Science, 309, 1559-1563 (2005).
  4. RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group) and the FANTOM Consortium:
    "Antisense Transcription in the Mammalian Transcriptome."
    Science, 309, 1564-1566 (2005).
  5. Shiraki, T., Kondo, S., Katayama, S., Waki, K., Kasukawa, T., Kawaji, H., Kodzius, R., Watahiki, A., Nakamura, M., Arakawa, T., Fukuda, S., Sasaki, D., Podhajska, A., Harbers, M., Kawai, J., Carninci, P., and Hayashizaki, Y.:
    "Cap analysis gene expression for high-throughput analysis of transcriptional starting point and identification of promoter usage."
    Proc Natl Acad Sci USA100, 15776-15781 (2003).
  6. The FANTOM Consortium and The RIKEN Genome Exploration Research Group Phase I & II Team:
    "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNA."
    Nature, 420, 563-573 (2002).
  7. The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium:
    "Functional annotation of 21,076 sequenced mouse cDNAs prepared from full-length enriched libraries."
    Nature, 409, 685-690 (2001).

Members

Principal Investigator

Yoshihide HAYASHIZAKI
Chief Scientist

Staff Scientist

Jun KAWAI
Vice Chief Scientist
Piero CARNINCI
Senior Research Scientist
Masayoshi ITO
Senior Research Scientist
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