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Cellular Dynamics Laboratory
Naoko IMAMOTO
Chief Scientist
Naoko IMAMOTO (Ph.D.)
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Research Areas

In eukaryotic cells, most of genomic information is stored in the cell nucleus. The main subject of our laboratory is to understand the nucleocytoplasmic transport and organization of cell nucleus to uncover new aspects and principles on regulation and maintenance of nuclear function. Our current effort has been focused on dissecting the dynamic behavior of nucleocytoplasmic transport machinery, transport pathways, and functional relation between nuclear envelope and chromatin structure in the context of live cells and cell-free reconstituted systems. We are taking cell biological, molecular biological, and biochemical approaches coupled with newly developed imaging techniques.

Research Subject

  1. Analysis of nucleocytoplasmic transport as system
  2. Biogenesis of nuclear pore complex
  3. Chromosome seggregation and replication

Related links

  1. RIKEN Advanced Science Institute Website_Laboratories PageNew Window
  2. Individual Website Laboratory PageNew Window

Press release

August 16,2010
Formation of nuclear membrane cavity complex directed by cell cycle engine

RIKEN RESEARCH

October 15,2010
Improving pore 'vision
The formation of nuclear pores in dividing human cells is being illuminated by new visualization techniques New Window
July 11,2008
Transport proteins make special deliveries
Two proteins responsible for ushering molecular cargo into the nucleus also play an unexpected role in making targeted deliveries during cell division New Window

List of Selected Publications

  1. Kose S, Furuta M., Imamoto N.:
    "Hikeshi, a nuclear import carrier for Hsp70s, protects cells from heat-shock induced nuclear damage"
    Cell, in press
  2. Clever M, Funakoshi T, Mimura Y., Takagi M, Imamoto N.:
    "The Nucleoporin ELYS/Mel28 regulates nuclear envelope subdomain formation in HeLa cell. "
    Nucleus, in press
  3. Funakoshi, T., Clever, M., Watanabe, A., Imamoto, N.:
    "Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase NPC assembly"
    Mol. Biol. Cell. 7: 1058-1069 (2011)
  4. Maeshima K, Iino H, Hihara S, Funakoshi T, Watanabe A, Nishimura M, Nakatomi R, Yahata K, Imamoto F, Hashikawa T, Yokota H, Imamoto N.:
    "Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase."
    Nat Struct Mol Biol. 17, 1065-1071. (2010)
  5. Iino H, Maeshima K, Nakatomi R, Kose S, Hashikawa T, Tachibana T, Imamoto N.:
    "Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells."
    Genes Cells Jun; 15 (6) : 647-60. (2010)
  6. Tahara, K,, Takagi, M., Ohsugi, M., Sone,T., Maeshima, K., Horiuchi,Y., Tokai-Nishizumi, N., Fumiko Nishiumi, F., Imamoto, F., Yamamoto, T., Kose, S. and Imamoto, N.:
    "Importin β and small GTPase Ran mediates the chromosome loading of human chromokinesin KID."
    J. Cell Biol, 180, 493-506 (2008).
  7. Tokunaga M, Imamoto N, Sakata-Sogawa K.:
    "Highly inclined thin illumination enables clear single-molecule imaging in cells."
    Nature Methods. 5, 159-161. (2008)
  8. Funakoshi, T., Maeshima, K., Yahata, K., Imamoto, F., Sugano, S., and Imamoto, N.:
    "TWO DISTINCT HUMAN POM121 GENES: Requirement for the formation of nuclear pore complexes."
    FEBS Lett, 581(25):4910-6. (2007)
  9. Maeshima, K., Yahata, K., Sasaki, Y., Nakatomi, R., Tachibana, T., Hashikawa, T., Imamoto,, F., and Imamoto, N.:
    "Cell cycle-dependent dynamics of nuclear pores: pore-free islands and lamins."
    J. Cell Sci., 119, 4442-4451 (2006)
  10. Kose, S., Furuta,M., Koike,M., Yoneda, Y., and Imamoto, N.:
    "The 70kDa heat-shock cognate protein facilitates the nuclear export of the import receptors"
    J. Cell Biol., 171, 19-25 (2005).

Members

Principal Investigator

Naoko IMAMOTO
Chief Scientist

Staff Scientist

Takeshi MIZUNO
Senior Research Scientist
Shingo KOSE
Senior Research Scientist
Masatoshi TAKAGI
Senior Research Scientist
Makoto KIMURA
Senior Research Scientist
Tomoko FUNAKOSHI
ASI Research Scientist

Postdoctoral Fellow

Yasuhiro MIMURA
Postdoctoral Researcher
Hiroshi MAMADA
Postdoctoral Researcher

Student Trainee

Yuko NISHIYAMA
Junior Research Associate
Yuumi ODA
Junior Research Associate

Technical Assistant

Ai WATANABE
Technical Staff I
Shouko MOTOHASHI
Technical Staff I
Ai KAMETAKA
Technical Staff I
Atsuko TAGUCHI
Technical Staff I