Cellular Dynamics Laboratory
In eukaryotic cells, most of genomic information is stored in the cell nucleus. The main subject of our laboratory is to understand the nucleocytoplasmic transport and organization of cell nucleus to uncover new aspects and principles on regulation and maintenance of nuclear function. Our current effort has been focused on dissecting the dynamic behavior of nucleocytoplasmic transport machinery, transport pathways, and functional relation between nuclear envelope and chromatin structure in the context of live cells and cell-free reconstituted systems. We are taking cell biological, molecular biological, and biochemical approaches coupled with newly developed imaging techniques.
- Analysis of nucleocytoplasmic transport as system
- Biogenesis of nuclear pore complex
- Chromosome seggregation and replication
- August 16,2010
- Formation of nuclear membrane cavity complex directed by cell cycle engine
- Kose S, Furuta M., Imamoto N.:
"Hikeshi, a nuclear import carrier for Hsp70s, protects cells from heat-shock induced nuclear damage"
Cell, in press - Clever M, Funakoshi T, Mimura Y., Takagi M, Imamoto N.:
"The Nucleoporin ELYS/Mel28 regulates nuclear envelope subdomain formation in HeLa cell. "
Nucleus, in press - Funakoshi, T., Clever, M., Watanabe, A., Imamoto, N.:
"Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase NPC assembly"
Mol. Biol. Cell. 7: 1058-1069 (2011) - Maeshima K, Iino H, Hihara S, Funakoshi T, Watanabe A, Nishimura M, Nakatomi R, Yahata K, Imamoto F, Hashikawa T, Yokota H, Imamoto N.:
"Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase."
Nat Struct Mol Biol. 17, 1065-1071. (2010) - Iino H, Maeshima K, Nakatomi R, Kose S, Hashikawa T, Tachibana T, Imamoto N.:
"Live imaging system for visualizing nuclear pore complex (NPC) formation during interphase in mammalian cells."
Genes Cells Jun; 15 (6) : 647-60. (2010) - Tahara, K,, Takagi, M., Ohsugi, M., Sone,T., Maeshima, K., Horiuchi,Y., Tokai-Nishizumi, N., Fumiko Nishiumi, F., Imamoto, F., Yamamoto, T., Kose, S. and Imamoto, N.:
"Importin β and small GTPase Ran mediates the chromosome loading of human chromokinesin KID."
J. Cell Biol, 180, 493-506 (2008). - Tokunaga M, Imamoto N, Sakata-Sogawa K.:
"Highly inclined thin illumination enables clear single-molecule imaging in cells."
Nature Methods. 5, 159-161. (2008) - Funakoshi, T., Maeshima, K., Yahata, K., Imamoto, F., Sugano, S., and Imamoto, N.:
"TWO DISTINCT HUMAN POM121 GENES: Requirement for the formation of nuclear pore complexes."
FEBS Lett, 581(25):4910-6. (2007) - Maeshima, K., Yahata, K., Sasaki, Y., Nakatomi, R., Tachibana, T., Hashikawa, T., Imamoto,, F., and Imamoto, N.:
"Cell cycle-dependent dynamics of nuclear pores: pore-free islands and lamins."
J. Cell Sci., 119, 4442-4451 (2006) - Kose, S., Furuta,M., Koike,M., Yoneda, Y., and Imamoto, N.:
"The 70kDa heat-shock cognate protein facilitates the nuclear export of the import receptors"
J. Cell Biol., 171, 19-25 (2005).
Principal Investigator
- Naoko IMAMOTO
- Chief Scientist
Staff Scientist
- Takeshi MIZUNO
- Senior Research Scientist
- Shingo KOSE
- Senior Research Scientist
- Masatoshi TAKAGI
- Senior Research Scientist
- Makoto KIMURA
- Senior Research Scientist
- Tomoko FUNAKOSHI
- ASI Research Scientist
Postdoctoral Fellow
- Yasuhiro MIMURA
- Postdoctoral Researcher
- Hiroshi MAMADA
- Postdoctoral Researcher
Student Trainee
- Yuko NISHIYAMA
- Junior Research Associate
- Yuumi ODA
- Junior Research Associate
Technical Assistant
- Ai WATANABE
- Technical Staff I
- Shouko MOTOHASHI
- Technical Staff I
- Ai KAMETAKA
- Technical Staff I
- Atsuko TAGUCHI
- Technical Staff I

