Ariadne, a unique web-based database search engine, correlates tandem mass spectra of sample RNA nucleolytic fragments with an RNA nucleotide sequence in a DNA/RNA sequence database, thereby allowing tandem mass spectrometry (MS/MS)-based identification of RNA in biological samples. Figure shows a typical search result obtained by the analysis of the doubly charged ion with m/z 1282.7, identified as the fragment 5'-OH-mAUCCACAG-2',3'-cyclic phosphate-3' spanning nucleotides 76-83 in the tRNAPhe1 sequence.
url: http://ariadne.riken.jp/