LSA Technology Development Group

Project Director
Harukazu SUZUKI (Ph.D.)

Deputy Project Director
Piero CARNINCI (Ph.D.)
Deputy Project Director
Masanori SUZUKI
LSA Technology Development Group implements the development, introduction, and standardization of the technologies, especially those for next generation sequencers, which constitute the Life Science Accelerator (LSA). The group maintains a close connection with the LSA System Development Group, Functional Genomics Technology Team, and the LSA Bioinformatics Core Facility in order to contribute to the leading research of the Omics Science Center. LSA technologies include exploration of novel pathways in gene expression regulation, development of various research tools by utilizing genomics resources, technology development for detecting genome variation, technology development for analysis of intra-gene interaction, development of various molecular libraries subjected to the LSA, and systematic proteome analysis.
A special task of this group is to achieve large-scale operations, at the level of the FANTOM (Functional ANnoTation Of Mammalian genome) projects, where technologies are scaled up in throughput and broadly tested on some yet unsolved biological problem. The FANTOM international meetings have been held five times and produced fruitful results as follows:
FANTOM1-3: The consortium developed an effective system for functional gene annotation (FANTOM1), and analyzed more than 60,770 full-length mouse cDNAs, which was published in a special issue of Nature on the decoding of the mouse genome (FANTOM2). In the FANTOM3, we published a couple of papers in the "RNA special issue" of Science in 2005, where we reported comparable existence of long non-coding RNA as well as protein coding RNA as "Discovery of new RNA Continent".
FANTOM4: We aimed to elucidate the transcriptional regulatory networks to understand biological phenomena at the molecular level. The development of the network analysis methods and findings of novel RNAs were published in three papers in the 'Nature genetics' special issue. Other results were published in the FANTOM special issue of 'Genome Biology', and results of the protein-protein interaction analyses of transcription factors in 'Cell'.
FANTOM5: We succeeded to develop high quantitative CAGE technology for application to the next generation sequencers. Based on this technology, promoter-centered large-scale transcriptome analyses are going on for various cell types in human and mouse.
Furthermore, as the application of above mentioned transcriptional regulation analysis, we developed a novel factor to promote pluripotency in mouse iPS cells, and direct cell conversion technology from human fibroblast cells.
- Development of the Life Science Accelerator
- Gene expression regulation network
- T Suzuki, M Nakano-Ikegaya, H Yabukami-Okuda, M De Hoon, J Severin, S Saga-Hatano, J W. Shin, A Kubosaki, C Simon, Y Hasegawa, Y Hayashizaki, H Suzuki,:
"Reconstruction of Monocyte Transcriptional Regulatory Network Accompanies Monocytic Functions in Human Fibroblasts."
PLoS One (2012 accepted) - Hasegawa Y, Takahashi N, Forrest AR, Shin JW, Kinoshita Y, Suzuki H, Hayashizaki Y.:
"CC chemokine ligand 2 and leukemia inhibitory factor cooperatively promote pluripotency in mouse induced pluripotent cells."
Stem Cells. 29(8):1196-205 (2011) - Ravasi T, Suzuki H, Cannistraci CV, Katayama S, Bajic VB, Tan K, Akalin A, Schmeier S, Kanamori-Katayama M, Bertin N, Carninci P, Daub CO, Forrest AR, Gough J, Grimmond S, Han JH, Hashimoto T, Hide W, Hofmann O, Kamburov A, Kaur M, Kawaji H, Kubosaki A, Lassmann T, van Nimwegen E, MacPherson CR, Ogawa C, Radovanovic A, Schwartz A, Teasdale RD, Tegnér J, Lenhard B, Teichmann SA, Arakawa T, Ninomiya N, Murakami K, Tagami M, Fukuda S, Imamura K, Kai C, Ishihara R, Kitazume Y, Kawai J, Hume DA, Ideker T, Hayashizaki Y.:
"An atlas of combinatorial transcriptional regulation in mouse and man."
Cell. 140(5), 744-752 (2010) - A Forrest, M Kanamori, Y Tomaru, T Lassmann, N Ninomiya, Y Takahashi, MJ De Hoon, A Kubosaki, A Kaiho, M Suzuki, J Yasuda, Y Hayashizaki, DA Hume, H Suzuki.:
"Induction of microRNAs mir-155, mir-222, mir-424 and mir-503, promotes monocytic differentiation through combinatorial regulation."
Leukemia, 24, 1-7 (2009) - H Suzuki, A Forrest, E van Nimwegen, CO Daub, P Balwierz, KM Irvine, T Lassmann, T Ravasi, Y Hasegawa, MJ De Hoon, S Katayama, K Schroder, P Carninci, Y Tomaru, M Kanamori, A Kubosaki, A Akalin, Y Ando, E Arner, M Asada, H Asahara, T Bailey, VB Bajic, D Bauer, A Beckhouse, N Bertin, J Björkegren, F Brombacher, E Bulger, A Chalk, J Chiba, N Cloonan, A Dawe, J Dostie, P Engstrom, M Essack, GJ Faulkner, J Fink, D Fredman, K Fujimori, M Furuno, T Gojyobori, J Gough, S Grimmond, M Gustafsson, M Hashimoto, T Hashimoto, M Hatakeyama, S Heinzel, W Hide, O Hofmann, M Hornquist, L Huminiecki, K Ikeo, N Imamoto, S Inoue, Y Inoue, R Ishihara, T Iwayanagi, A Jacobsen, M Kaur, H Kawaji, M Kerr, R Kimura, S Kimura, Y Kimura, H Kitano, H Koga, T Kojima, S Kondo, T Konno, A Krogh, A Kruger, A Kumar, B Lenhard, A Lennartsson, M Lindow, M Lizio, CR MacPherson, N Maeda, C Maher, M Maqungo, JC Mar, N Matigian, H Matsuda, J Mattick, S Meier, S Miyamoto, E Miyamoto-Sato, K Nakabayashi, Y Nakachi, M Nakano, S Nygaard, T Okayama, Y Okazaki, H Okuda-Yabukami, V Orlando, J Otomo, M Pachkov, N Petrovsky, C Plessy, J Quackenbush, A Radovanovic, M Rehli, R Saito, A Sandelin, S Schmeier, C Schonbach, A Schwartz, C Semple, M Sera, J Severin, K Shirahige, C Simons, G St. Laurent, M Suzuki, T Suzuki, M Sweet, RJ Taft, S Takeda, Y Takenaka, K Tan, M Taylor, R Teasdale, J Tegnér, S Teichmann, E Valen, C Wahlestedt, K Waki, A Waterhouse, C Wells, O Winther, L Wu, K Yamaguchi, H Yanagawa, J Yasuda, M Zavolan, DA Hume, T Arakawa, S Fukuda, K Imamura, C Kai, A Kaiho, T Kawashima, C Kawazu, Y Kitazume, M Kojima, H Miura, K Murakami, M Murata, N Ninomiya, H Nishiyori, S Noma, C Ogawa, T Sano, C Simon, M Tagami, Y Takahashi, J Kawai, Y Hayashizaki.:
"The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line"
Nature Genetics, 41 (5), 553-562 (2009) - GJ Faulkner, Y Kimura, CO Daub, S Wani, C Plessy, KM Irvine, K Schroder, N Cloonan, AL Steptoe, T Lassmann, K Waki, N Hornig, T Arakawa, H Takahashi, J Kawai, A Forrest, H Suzuki, Y Hayashizaki, DA Hume, V Orlando, S Grimmond, P Carninci,:
"The regulated retrotransposon transcriptome of mammalian cells"
Nat Genet, 41(5), 563-571 (2009) - RJ Taft, E Glazov, N Cloonan, C Simons, S Stephen, GJ Faulkner, T Lassmann, A Forrest, S Grimmond, K Schroder, KM Irvine, T Arakawa, M Nakamura, A Kubosaki, K Hayashida, C Kawazu, M Murata, H Nishiyori, S Fukuda, J Kawai, CO Daub, DA Hume, H Suzuki, V Orlando, P Carninci, Y Hayashizaki, J Mattick.:
"The Transcriptional Landscape of the Mammalian Genome.Tiny RNAs associated with transcription start sites in animals"
Nat Genet, 41:5, 572-578 (2009) - Kubosaki A, Tomaru Y, Tagami M, Arner E, Miura H, Suzuki T, Suzuki M, Suzuki H, Hayashizaki Y.
"Genome-wide investigation of in vivo EGR-1 binding sites in monocytic differentiation."
Genome Biol.10(4), R41 (2009) - Mitani Y, Lezhava A, Kawai Y, Kikuchi T, Oguchi-Katayama A, Kogo Y, Itoh M, Miyagi T, Takakura H, Hoshi K, Kato C, Arakawa T, Shibata K, Fukui K, Masui R, Kuramitsu S, Kiyotani K, Chalk A, Tsunekawa K, Murakami M, Kamataki T, Oka T, Shimada H, Cizdziel PE, Hayashizaki Y.
"Rapid SNP diagnostics using asymmetric isothermal amplification and a new mismatch-suppression technology."
Nat Methods 4(3) 257-262 (2007) - Carninci P, Sandelin A, Lenhard B, Katayama S, Shimokawa K, Ponjavic J, Semple CA, Taylor MS, Engström PG, Frith MC, Forrest AR, Alkema WB, Tan SL, Plessy C, Kodzius R, Ravasi T, Kasukawa T, Fukuda S, Kanamori-Katayama M, Kitazume Y, Kawaji H, Kai C, Nakamura M, Konno H, Nakano K, Mottagui-Tabar S, Arner P, Chesi A, Gustincich S, Persichetti F, Suzuki H, Grimmond SM, Wells CA, Orlando V, Wahlestedt C, Liu ET, Harbers M, Kawai J, Bajic VB, Hume DA, Hayashizaki Y.
"Genome-wide analysis of mammalian promoter architecture and evolution."
Nat Genet. 38: 626-35. (2006)
Principal Investigator
- Harukazu SUZUKI
- Project Director
Staff Scientist
- Masanori SUZUKI
- Deputy Project Director
- Piero CARNINCI
- Deputy Project Director
LSA Technology Development Unit
Principal Investigator
- Yoshihide HAYASHIZAKI
- Unit Leader
Staff Scientist
- Yoshiyuki NAGAI
- Senior Scientist
- Toshihisa ISHIKAWA
- Senior Scientist
- Masayoshi ITO
- Senior Scientist
- Kengo USUI
- Jae Woo SHIN
- Yuki HASEGAWA
- Research Scientist
- Takeshi HANAMI
- Research Scientist
- Atsutaka KUBOSAKI
- Research Scientist
- Takahiro SOMA
- Research Scientist
- Yuki TANAKA
- Research Scientist
- Yasuhiro TOMARU
- Research Scientist
- Hiromi SUEKI
- Technical Scientist
- Christophe SIMON
- Technical Scientist
- Mitsuyoshi MURATA
- Technical Scientist
Postdoctoral Fellow
- Takahiro SUZUKI
- Postdoctoral Researcher
- Yusuke OGUCHI
- Postdoctoral Researcher
Student Trainee
- Yutaka NEGISHI
- Junior Research Associate
- Ryota HASEGAWA
- Junior Research Associate
Technical Assistant
- Kazuhito NOMURA
- Technical Staff I
- Miki KOJIMA
- Technical Staff I
- Noriko FUKUDA
- Technical Staff I
- Yuri ISHIZU
- Technical Staff I
- Ai KAIHO
- Technical Staff I
- Yasumasa KIMURA
- Technical Staff I
- Chizuru SUZUKI
- Technical Staff I
- Mami IKEGAMI
- Technical Staff I
- Sachi KATO
- Technical Staff I
- Haruka OKUDA
- Technical Staff I
- Naoko TAKAHASHI
- Technical Staff I
- Jean-Etienne MORLIGHEM
- Technical Staff I
- Satomi HATANO
- Technical Staff I
- Saori GODA
- Technical Staff I
- Hiroko KINOSHITA
- Technical Staff I
- Tsukasa KOUNO
- Technical Staff I
Omics Molecular Interaction Research Unit
Principal Investigator
- Harukazu SUZUKI
- Unit Leader
Staff Scientist
- Sugata ROY
- Research Scientist
Omics Resource Development Unit
Principal Investigator
- Piero CARNINCI
- Unit Leader
Omics Expression Research Unit
Principal Investigator
- Harukazu SUZUKI
- Unit Leader
Genome Profiling Technology Unit
Principal Investigator
- Alistair Raymond Russell FORREST
- Unit Leader