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Laboratory for Neuronal Growth Mechanisms
Hiroyuki KAMIGUCHI
Laboratory Head
Hiroyuki KAMIGUCHI (M.D.,Ph.D.)
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Research Areas

The development of functional neuronal networks relies on the spatial accuracy of axon extension. Each extending axon is tipped by a growth cone that can interpret extracellular "guidance cues" and navigate the axon along the correct path. After neuronal networks have been developed, injury to the adult central nervous system induces the formation of barriers to regenerating axons. It is well known that growth cones use similar mechanisms for their navigation during development and regeneration. Our aim is to investigate growth cone navigation with particular emphasis on the roles of guidance cues, intracellular signals, cytoskeleton, adhesion machinery and membrane dynamics. These revelations will help elucidate the fundamental principles of axon tract formation and establish the molecular and cellular biological basis for axon tract repair.

Research Subject

  1. Intracellular signals that regulate axon growth and guidance
  2. Cytoskeletal and membrane dynamics for growth cone navigation
  3. Lipids and sugars that control neuronal network formation

Related links

  1. RIKEN Brain Science Institute Website_Laboratories PageNew Window

Press release

May 13, 2010
Mechanism discovered by which regeneration inhibitors bypass neuron protrusions
February 02, 2010
Front end of brain cell discovered to rotate clockwise
July 10, 2009
Substance identified that regulates growth direction of neuron protrusion

RIKEN RESEARCH

July 23, 2010
A turning point for young neurons
Inward flow of membrane material is critical for repulsive growth cone turningNew Window
April 09, 2010
Twisting in the right direction
Spontaneous rotating movements cause growing nerve fibers to turn to the rightNew Window
October 23, 2009
Helping neurons find their way
Variations in the spatial distribution of cellular signaling molecules provide the information needed to steer neuron growth within the brainNew Window
October 12, 2007
How does a neuronal process reach its destination?New Window
February 23, 2007
Steering mechanism for nerve circuit formation revealed
A recent study extends our understanding of how nerve cells reach out towards attractive stimuliNew Window

List of Selected Publications

  1. Tojima, T., Hines, J.H., Henley, J.R., and Kamiguchi, H.:
    "Second messengers and membrane trafficking direct and organize growth cone steering"
    Nature Reviews Neuroscience 12, 191-203 (2011)
  2. Akiyama, H. and Kamiguchi, H.:
    "Phosphatidylinositol 3-kinase facilitates microtubule-dependent membrane transport for neuronal growth cone guidance."
    The Journal of Biological Chemistry 285, 41740-41748 (2010)
  3. Tojima, T., Itofusa, R., and Kamiguchi, H.:
    "Asymmetric clathrin-mediated endocytosis drives repulsive growth cone guidance."
    Neuron 66, 370-377 (2010)
  4. Tamada, A., Kawase, S., Murakami, F., and Kamiguchi, H.:
    "Autonomous right-screw rotation of growth cone filopodia drives neurite turning."
    The Journal of Cell Biology 188, 429-441(2010)
  5. Akiyama, H., Matsu-ura, T., Mikoshiba, K., and Kamiguchi, H.:
    "Control of neuronal growth cone navigation by asymmetric inositol 1,4,5-trisphosphate signals."
    Science Signaling 2, ra34 (2009)
  6. Tojima, T., Itofusa, R., and Kamiguchi, H.:
    "The nitric oxide-cyclic GMP pathway controls the directional polarity of growth cone guidance via modulating cytosolic Ca2+ signals."
    The Journal of Neuroscience 29, 7886-7897 (2009)
  7. Tojima, T., Akiyama, H., Itofusa, R., Li, Y., Katayama, H., Miyawaki, A., and Kamiguchi, H.:
    "Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone."
    Nature Neuroscience 10, 58-66 (2007)
  8. Ooashi, N., Futatsugi, A., Yoshihara, F., Mikoshiba, K., and Kamiguchi, H.:
    "Cell adhesion molecules regulate Ca2+-mediated steering of growth cones via cyclic AMP and ryanodine receptor type3."
    The Journal of Cell Biology 170, 1159-1167 (2005)
  9. Nishimura, K., Yoshihara, F., Tojima, T., Ooashi, N., Yoon, W., Mikoshiba, K., Bennett, V., and Kamiguchi, H.:
    "L1-dependent neuritogenesis involves ankyrinB that mediates L1-CAM coupling with retrograde actin flow."
    The Journal of Cell Biology 163, 1077-1088 (2003)
  10. Nakai, Y. and Kamiguchi, H.:
    "Migration of nerve growth cones requires detergent-resistant membranes in a spatially defined and substrate-dependent manner."
    The Journal of Cell Biology 159, 1097-1108 (2002)

Members

Principal Investigator

Hiroyuki KAMIGUCHI
Laboratory Head

Members

Takuro TOJIMA
Research Scientist
Adam Tsuda GUY
Research Scientist
Fumitaka WADA
Research Scientist
Carmen CHAN
Research Scientist
Hiroki AKIYAMA
Special Postdoctoral Researcher
Tetsuko FUKUDA
Technical Staff I
Asuka NAKATA
Technical Staff I
Natsuko MATSUZAKI
Technical Staff I
Rurika ITOFUSA
Technical Staff I
Mariko INOUE
Technical Staff I
Noriko OOASHI
Technical Staff I
Mineko TOMOMURA
Senior Visiting Scientist
Tomoharu KUBOYAMA
Visiting Scientist
Sayori YAGI
Part-time Staff
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