X-ray Structural Analysis Research Team
Team Leader
Toshiro ODA (Ph.D.)
To understand the mechanism of molecular machinary in the cytoskeleton such as actin motor, we study the structure and its dynamics from viewpoints of structural biology. We interdisciplinarily use the X-ray fiber diffraction method, the small angle scattering method and the bio-crystallography at SPring-8 beam line to solve the structures of molecular machinary consiting of multiple proteins. Also, we use the neutron inelastic scattering experimentally to detect the structural dynamics. We complementally use the molecular simulation at the immeasurable time scale. The aim of our taem is to understand physiological event deriven by some moleular machinary such as the muscle contrauction and the hearing processs.
- Structure and dynamics of F-actin
- An initial process of actin polymerization and nucleation factors
- Actin function by the use of actin mutant
- Dvelopement of X-ray fiber diffraction analysis
- T. Ito, A. Narita, T. Hirayama, M. Taki, S. Iyoshi, Y. Yamamoto, Y. Maéda and T. Oda.:
"Human Spire Interacts with the Barbed End of the Actin Filament."
J Mol. Biol. 406: 416-426. - S. K. Tewarya, T. Oda, A. Kendall, W. Bian, G. Stubb, S.-M. Wonga and K. Swaminathana.:
"Structure determination of Hibiscus latent Singapore virus by X-ray fiber diffraction: a non-conserved His122 contributes to coat protein stability."
J Mol Biol. 406:516-526. (2011) - T. Oda and Y. Maéda.:
"Multiple Conformations of F-actin."
Structure. 18:761-767. (2010) - T. Oda, M. Iwasa, T. Aihara, Y. Maeda, A. Narita.:
"The nature of the globular- to fibrous-actin transition."
Nature. 457:441-445 (2009) - T. Splettstoesser , F. Noe, T. Oda, JC.Smith.:
"Nucleotide-dependence of G-actin conformation from multiple molecular dynamics simulations and observation of a putatively polymerization-competent superclosed state."
Proteins 180, 1188-1195 (2009).
Principal Investigator
- Toshiro ODA
- Team Leader
Staff Scientist
- Tomoki AIHARA
- Research Scientist