Laboratories
Three-dimensional Microscopy Research Team
Teruhisa HIRAI
Team Leader
Teruhisa HIRAI (Ph.D.)
mail

Research Areas

Our goal is to understand the structure and mechanism of transport proteins and to reveal the physiological role of transport system from the structural point of view. Reliable method to make two-dimensional crystal is desired to understand native conformation of membrane proteins at high resolution. To understand the mechanism of transporters appropriate methods including various spectroscopic techniques need to be applied. Furthermore we will push forward the method of electron tomography to figure out the overall transport system.

Research Subject

  1. Two-dimensional crystallization of membrane proteins for electron crystallography
  2. Analysis of structure and mechanism of transporters
  3. Structural analysis of membrane transport system by electron tomography

Related links

  1. RIKEN SPring-8 Center Website_Laboratories PageNew Window

Press release

January 22, 2010
Structure of "Band-3" metabolic erythrocyte sensor identified

RIKEN RESEARCH

April 09,2010
Insights to air supply
The structure of a key membrane protein expressed in red blood cells could reveal how oxygen supply to tissues is regulated New Window

List of Selected Publications

  1. Hirai, T., Hamasaki, N., Yamaguchi, T. and Ikeda, Y.:
    "Topology models of anion exchanger 1 that incorporate the anti-parallel V-shaped motifs found in the EM structure"
    Biochem. Cell Biol. (in print)
  2. Yamaguchi, T., Ikeda, Y., Abe, Y., Kuma, H., Kang, D., Hamasaki, N., and Hirai, T.:
    "Structure of the membrane domain of human erythrocyte anion exchanger 1 revealed by electron crystallography."
    J. Molecular Biology 397(1), 179-189 (2010)
  3. Hirai, T., Subramaniam, S., and Lanyi, J.K.:
    "Structural snapshots of conformational changes in a seven-helical membrane protein: lessons from bacteriorhodopsin."
    Curr. Opin. Struct. Biol. 19(4), 433-439 (2009)
  4. Hirai, T., and Subramaniam, S.:
    "Protein conformational changes in the bacteriorhodopsin photocycle: comparison of findings from electron and X-ray crystallographic analyses."
    PLoS One 4(6):e5769 (2009)
  5. Hirai, T., Mitsuoka, K., Kidera, A., and Fujiyoshi, Y.:
    "Simulation of charge effects on density maps obtained by high-resolution electron crystallography."
    J. Electron Microsc. 56(4), 131-140 (2007)
  6. Hirai, T., and Subramaniam, S.:
    "Structure and transport mechanism of the bacterial oxalate transporter OxlT."
    Biophys. J., 87, 3600-3607 (2004)
  7. Hirai, T., and Subramaniam, S.:
    "Structural insights into the mechanism of proton pumping by bacteriorhodopsin."
    FEBS Lett., 545(1), 2-8 (2003)
  8. Hirai, T., Heymann, J., Maloney, P.C., and Subramaniam, S.:
    "A structural model for 12-helix transporters belonging to the Major Facilitator Superfamily."
    J. Bacteriology, 185(5), 1712-1718 (2003)
  9. Hirai, T., Heymann, J., Shi, D., Sarker, R., Maloney, P.C., and Subramaniam, S.: "Three-dimensional structure of a bacterial oxalate transporter." Nature Struct. Biol. 9(8), 597-600 (2002)

Members

Principal Investigator

Teruhisa HIRAI
Team Leader

Staff Scientist

Yohei IKEDA
Research Scientist