Bio-multisome Research Team
Team Leader
Atsuo MIYAZAWA (Ph.D.)
The aim of our research is to elucidate the advanced molecular mechanism of a functional complex of proteins in vivo (bio-multisome) from a viewpoint of structural biology in detail using microscopy freely. It has become clear the importance of bio-multisome which takes on the several events in cells. Therefore, we would establish the new technical approach that can elucidate the wide range of three-dimensional structures which overlook the configuration of bio-multisome reaching a cellular level from an atomic level, by the development of microscopic analyses such as electron crystallography, single particle analysis, electron tomography and light microscopy. These experimental attempts will provide critical insights into the dynamic structure, localization, and function of the bio-multisome in living cells.
- Structural analysis of membrane protein
- Study of the scaffolding proteins at postsynapse
- Molecular labeling of intracellular proteins for electron microscopy
- Three-dimensional image analysis of proteins in the cell
- Miyazawa, A., Fujiyoshi, Y., and Unwin, N.:
"Structure and gating mechanism of the acetylcholine receptor pore"
Nature, 423, 949 (2003). - Yuko Fukunaga, Mamoru Matsubara, Rina Nagai and Atsuo Miyazawa.:
"The interaction between PSD-95 and Ca2+/calmodulin is enhanced by PDZ-binding proteins"
J. Biochem., 138(2), 177 (2005) - Nishino, Y., Yasunaga, T., and Miyazawa, A.:
"A genetically encoded metallothionein tag enabling efficient protein detection by electron microscopy"
J. Electron. Microsc., 56(3), 93 (2007) - Fukunaga, Y., Hirase, A., Kim, H., Wada, N., Nishino, Y., and Miyazawa, A.:
"Electron microscopic analysis of a fusion protein of postsynaptic desity-95 and metallothionein in cultured hippocampal neurons"
J. Electron Microsc., 56(4), 119 (2007) - Shimada, A., Niwa, H., Tsujita, K., Suetsugu, S., Nitta, K., Hanawa-Suetsugu, K., Akasaka, R., Nishino, Y., Toyama, M., Chen, L., Liu, Z.-J., Wang, B.-C., Yamamoto, M., Terada, T., Miyazawa, A., Tanaka, A., Sugano, S., Shirouzu, M., Nagayama, K., Takenawa.:
"Curved EFC/F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis"
Cell, 129, 761 (2007) - Aoyama, K., Takagi, T., Hirase, A., and Miyazawa, A.:
"STEM tomography for thick biological specimens"
Ultramicroscopy, 109, 70 (2008) - Shigemoto, K., Kubo, S., Jie, C., Hato, N., Abe, Y., Ueda, N., Kobayashi, H., Kameda, K., Mominoki, K., Miyazawa, A., Ishigami, A., Matsuda, S., and Maruyama, N.:
"Myasthenia gravis experimentally induced with muscle-specific kinase"
Annals of the New York Academy of Sciences, 1132, 93 (2008) - Mori, S., Kubo, S., Akiyoshi, T., Yamada, S., Miyazaki, T., Hotta, H., Desaki, J., Kishi, M., Konishi, T., Nishino, Y., Miyazawa, A., Maruyama, N. and Shigemoto, K.:
"Antibodies against muscle-specific kinase impair both presynaptic and postsynaptic functions in a murine model of myasthenia gravis"
American J. Pathology, 180(2), 798 (2012) - Machida, K., Masutani, M., Kobayashi, T., Mikami, S., Nishino, Y., Miyazawa, A. and Imataka, H.:
"Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells"
Protein Expression and Purification, 82, 61 (2012) - Noda, T., Sugita, Y., Aoyama, K., Hirase, A., Kawakami, E., Miyazawa, A., Sagara, H. and Kawaoka, Y.:
"Three-dimensional analysis of ribonucleoprotein complexes in influenza A virus"
Nature Communications, in press (2012)
Principal Investigator
- Atsuo MIYAZAWA
- Team Leader
Staff Scientist
- Yuri NISHINO
- Research Scientist
Postdoctoral Fellow
- Ai HIGASHIHARA
- Research Associate