Laboratories
Structural Glycobiology Team
Yoshiki YAMAGUCHI
Team Leader
Yoshiki YAMAGUCHI (D.Pharm.)
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Research Areas

In order to elucidate the functions of the glycans attached to proteins, it is important to analyze the glycans from the structural point of view. Our team aim to reveal the functions of glycans through the three-dimensional structures of glycoproteins and oligosaccharide-related proteins. We prepare the glycoconjugates samples with the techniques of organic synthesis and cell-engineering, and determine the three-dimensional structures using biophysical methods such as NMR or X-ray crystallography. Furthermore, we construct databases of oligosaccharide structures using bioinformatics-based approaches and decode the 'glycosignals' from the structural information. We particularly focus on disease-related glycoproteins, and develop diagnostic and therapeutic agents.

Related links

  1. RIKEN Advanced Science Institute Website_Laboratories PageNew Window

Research Subject

  1. Development of conformational analysis of glycans
  2. Recognition mode of glycan-binding molecules and their functions
  3. Construction of database on 3D structures of glycans

Press release

December 23,2010
3D structure of glycoprotein-capturing lectin OS-9 elucidated

RIKEN RESEARCH

October 21,2011
Recognizing pathogenic invaders
Elucidation of the structure of receptors that detect invading pathogens in moths could aid the diagnosis of infectious fungal diseases New Window

List of Selected Publications

  1. Subedi, G.P., Satoh, T., Hanashima, S., Ikeda, A., Nakada, H., Sato, R., Mizuno, M., Yuasa, N., Fujita-Yamaguchi, Y., and Yamaguchi, Y.:
    "Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector"
    Protein Expr. Purif. 82, 197-204 (2012)
  2. Matsumoto-Takasaki, A., Hanashima, S., Aoki, A., Yuasa, N., Ogawa, H., Sato, R., Kawakami, H., Mizuno, M., Nakada, H., Yamaguchi, Y., and Fujita-Yamaguchi, Y.:
    "Surface plasmon resonance and NMR analyses of anti Tn-antigen MLS128 monoclonal antibody binding to two or three consecutive Tn-antigen clusters"
    J. Biochem. (2011) [Epub ahead of print]
  3. Re, S., Miyashita, N., Yamaguchi, Y., and Sugita, Y.:
    "Structral diversity and changes in conformational equilibria of biantennary complex-type N-glycans in water revealed by replica-exchange molecular dynamics simulation"
    Biophys. J. 101, L44-6 (2011)
  4. Hanashima, S., Kato, K., and Yamaguchi, Y.:
    "13C-NMR quantification of proton exchange at LewisX hydroxyl groups in water"
    Chem. Comm. 47, 10800-10802 (2011)
  5. Fujita, M., Watanabe, R., Jaensch, N., Romanova-Michaelides, M., Satoh, T., Kato, M., Riezman, H., Yamaguchi, Y., Maeda, Y., and Kinoshita, T.:
    "Sorting of GPI-anchored proteins into ER exit sites by p24 proteins is dependent on remodeled GPI"
    J. Cell Biol. 194, 61-75 (2011)
  6. Kanagawa, M., Satoh, T., Ikeda, A., Adachi, Y., Ohno, N., and Yamaguchi, Y.:
    "Structural insights into recognition of triple-helical β-glucans by an insect fungal receptor"
    J. Biol. Chem. 286, 29158-29165 (2011)
  7. Satoh, T., Chen, Y, Hu, D., Hanashima, S., Yamamoto, K., and Yamaguchi, Y.:
    "Structural basis for oligosaccharide recognition of misfolded glycoproteins by OS-9 in ER-associated degradation"
    Mol. Cell 40, 905-916 (2010)
  8. Kanagawa, M., Satoh, T., Ikeda, A., Nakano, Y., Yagi, H., Kato, K., Kojima-Aikawa, K., and Yamaguchi, Y.:
    "Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related β-prism fold"
    Biochem. Biophys. Res. Commun. 404, 201-205 (2011)
  9. Yamaguchi, Y., and Kato, K.:
    "Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy"
    Methods Enzymol. 478, 305-322 (2010)
  10. Kato, K., Yamaguchi, Y., and Arata, Y.:
    "Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a model system"
    Prog. Nucl. Magn. Reson. Spectrosc. 56, 346-359 (2010)

Members

Principal Investigator

Yoshiki YAMAGUCHI
Team Leader

Staff Scientist

Shinya HANASHIMA
ASI Research Scientist
Mayumi KANAGAWA
ASI Research Scientist
Masamichi NAGAE
ASI Research Scientist

Student Trainee

Ganesh Prasad SUBEDI
International Program Associate

Technical Assistant

Masaki KATO
Technical Staff I
Akemi IKEDA
Technical Staff I
Akitsugu SUGA
Technical Staff I

Visiting Research Staff

Yutaro HAYASHI
Senior Visiting Scientist

Others

Jun UZAWA
Part-timer1
Noriko TANAKA
Part-timer2
Kana MATSUMOTO
Agency Staff