Structural Glycobiology Team
In order to elucidate the functions of the glycans attached to proteins, it is important to analyze the glycans from the structural point of view. Our team aim to reveal the functions of glycans through the three-dimensional structures of glycoproteins and oligosaccharide-related proteins. We prepare the glycoconjugates samples with the techniques of organic synthesis and cell-engineering, and determine the three-dimensional structures using biophysical methods such as NMR or X-ray crystallography. Furthermore, we construct databases of oligosaccharide structures using bioinformatics-based approaches and decode the 'glycosignals' from the structural information. We particularly focus on disease-related glycoproteins, and develop diagnostic and therapeutic agents.
- Development of conformational analysis of glycans
- Recognition mode of glycan-binding molecules and their functions
- Construction of database on 3D structures of glycans
- December 23,2010
- 3D structure of glycoprotein-capturing lectin OS-9 elucidated
- Subedi, G.P., Satoh, T., Hanashima, S., Ikeda, A., Nakada, H., Sato, R., Mizuno, M., Yuasa, N., Fujita-Yamaguchi, Y., and Yamaguchi, Y.:
"Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector"
Protein Expr. Purif. 82, 197-204 (2012) - Matsumoto-Takasaki, A., Hanashima, S., Aoki, A., Yuasa, N., Ogawa, H., Sato, R., Kawakami, H., Mizuno, M., Nakada, H., Yamaguchi, Y., and Fujita-Yamaguchi, Y.:
"Surface plasmon resonance and NMR analyses of anti Tn-antigen MLS128 monoclonal antibody binding to two or three consecutive Tn-antigen clusters"
J. Biochem. (2011) [Epub ahead of print] - Re, S., Miyashita, N., Yamaguchi, Y., and Sugita, Y.:
"Structral diversity and changes in conformational equilibria of biantennary complex-type N-glycans in water revealed by replica-exchange molecular dynamics simulation"
Biophys. J. 101, L44-6 (2011) - Hanashima, S., Kato, K., and Yamaguchi, Y.:
"13C-NMR quantification of proton exchange at LewisX hydroxyl groups in water"
Chem. Comm. 47, 10800-10802 (2011) - Fujita, M., Watanabe, R., Jaensch, N., Romanova-Michaelides, M., Satoh, T., Kato, M., Riezman, H., Yamaguchi, Y., Maeda, Y., and Kinoshita, T.:
"Sorting of GPI-anchored proteins into ER exit sites by p24 proteins is dependent on remodeled GPI"
J. Cell Biol. 194, 61-75 (2011) - Kanagawa, M., Satoh, T., Ikeda, A., Adachi, Y., Ohno, N., and Yamaguchi, Y.:
"Structural insights into recognition of triple-helical β-glucans by an insect fungal receptor"
J. Biol. Chem. 286, 29158-29165 (2011) - Satoh, T., Chen, Y, Hu, D., Hanashima, S., Yamamoto, K., and Yamaguchi, Y.:
"Structural basis for oligosaccharide recognition of misfolded glycoproteins by OS-9 in ER-associated degradation"
Mol. Cell 40, 905-916 (2010) - Kanagawa, M., Satoh, T., Ikeda, A., Nakano, Y., Yagi, H., Kato, K., Kojima-Aikawa, K., and Yamaguchi, Y.:
"Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related β-prism fold"
Biochem. Biophys. Res. Commun. 404, 201-205 (2011) - Yamaguchi, Y., and Kato, K.:
"Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy"
Methods Enzymol. 478, 305-322 (2010) - Kato, K., Yamaguchi, Y., and Arata, Y.:
"Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a model system"
Prog. Nucl. Magn. Reson. Spectrosc. 56, 346-359 (2010)
Principal Investigator
- Yoshiki YAMAGUCHI
- Team Leader
Staff Scientist
- Shinya HANASHIMA
- ASI Research Scientist
- Mayumi KANAGAWA
- ASI Research Scientist
- Masamichi NAGAE
- ASI Research Scientist
Student Trainee
- Ganesh Prasad SUBEDI
- International Program Associate
Technical Assistant
- Masaki KATO
- Technical Staff I
- Akemi IKEDA
- Technical Staff I
- Akitsugu SUGA
- Technical Staff I
Visiting Research Staff
- Yutaro HAYASHI
- Senior Visiting Scientist
Others
- Jun UZAWA
- Part-timer1
- Noriko TANAKA
- Part-timer2
- Kana MATSUMOTO
- Agency Staff

