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Laboratory for Organogenesis and Neurogenesis
Yoshiki SASAI
Group Director
Yoshiki SASAI
(M.D., Ph.D.)
mail

Research Areas

Vertebrate embryogenesis is a complicated process that involves a number of cell-cell interactions in emrbyonic tissues. This group in focusing on molecular mechnisms underlying cell-cell interaction (inductive process) in organogenesis of complex organs such as brain. Also, we attempt to establish scientific bases for therapeutic application of in vitro differentiation systems.

Research Subject

  1. Molecular mechanisms of fine positional information controlling brain development
  2. Molecular analysis on brain pattern formation by using ES cells
  3. Selective in vitro differentiation of cells and tissues for therapeutic application

Related links

  1. RIKEN Center for Developmental Biology Website_Laboratories PageNew Window

Press release

August 07, 2010
Researchers succeed in producing and transplanting cerebellum neurons from ES cells New Window
September 13,2010
Death dance reveals secrets of apoptosis in dissociated human ES cells
September 05, 2008
Knowing when to stop: New stability factor controls robustness of the dorsal-ventral axis
May 28,2007
Laboratory for Organogenesis and Neurogenesis of CDB succeeded in massive cell generation and the production of cerebral neural cells according to the development of a unique protocol for culturing cells from human embryonic stem cells.

RIKEN RESEARCH

July 01, 2011
Insights into the story of the eye
Cultures of embryonic stem cells can be coaxed to spontaneously develop into a mature, properly organized retina New Window
May 13, 2011
The beginnings of the brain
A single protein is sufficient to switch on the various genes that kick off the development of the embryonic nervous system New Window
December 24,2010
Keeping brain development in focus
A newly characterized protein promotes embryonic brain formation by hiding a receptor with the potential to undermine this process New Window
November 05,2010
Replacing faulty neurons
An effective method for generating cerebellar neurons could lead to new treatments for movement disorders New Window
October 08,2010
Rocking the death dance
Identification of the mechanism that triggers suicide in dissociated human stem cells should assist their development as therapeutics New Window
January 09, 2009
Balancing act
A recently discovered protein works behind the scenes to confer much-needed stabilization to an essential developmental pathway New Window
December 5, 2008
The right mix for neuron culture
Eliminating signaling molecules from tissue culture results in the generation of hypothalamic neurons from embryonic stem cells New Window
November 28,2008
Rebuilding the brain
A method for deriving complex neuronal tissues from embryonic stem cells could yield major benefits for clinical research and the development of new therapeutics New Window
October 16,2008
Guiding the decision-making process
Identification of a novel protein involved in embryonic development leads to new insights into the first stage of neural development New Window
August 03,2007
Embryos back to front
Several protein interactions help to establish the front and back sides of an embryo New Window

List of Selected Publications

  1. Suga, H., Kadoshima, T., Minaguchi, M., Ohgushi, M., Soen, M.,Nakano, T., Takata, N., Wataya, T., Muguruma, K., Miyoshi, H., Yonemura, S., Oiso, Y. & Sasai, Y.:
    "Self-formation of functional adeno-hypophysis in three-dimensional culture."
    Nature 480, 57-62 (2011)
  2. Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T. and Sasai, Y.:
    "Self-organizing optic-cup morphogenesis in three-dimensional culture. "
    Nature 472, 51-56 .(2011)
  3. Kamiya, D., Banno, S., Sasai, N., Ohgushi, M., Watanabe, K., Kawada, M., Yakura, R., Jakt , L.M., Nishikawa, S. and Sasai. Y.:
    "Intrinsic transition of ES cell differentiation into neural progenitors."
    Nature 470, 503-509 (2011)
  4. Aramaki, T., Sasai, N., Yakura, R., Sasai, Y.:
    "Jiraiya attenuates BMP signaling by interfering with type II BMP receptors in neuroectodermal patterning."
    Dev Cell 19, 547-561 (2010).
  5. Muguruma, K., Nishiyama, A., Ono, Y., Miyawaki, H., Mizuhara, E., Hori, S., Kakizuka, A., Obata, K., Yanagawa, Y., Hirano, T., Sasai, Y.:
    "Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells."
    Nature Neuroscience 13, 1171-1181 (2010).
  6. Ohgushi, M., Matsumura, M., Eiraku, M., Murakami, K., Aramaki, T., Nishiyama, A., Muguruma, K., Nakano, T., Suga, H., Ueno, M., Ishizaki, T., Suemori, H., Narumiya, S., Niwa, H. and Sasai, Y.:
    "Molecular Pathway and Cell State Responsible for Dissociation-Induced Apoptosis in Human Embryonic Stem Cells."
    Cell Stem Cell 7, 225-39 (2010)
  7. Takai, A., Inomata, H., Arakawa, A., Yakura, R., Matsuo-Takasaki, M., Sasai, Y.:
    "Anterior neural development requires Del1, a matrix-associated protein that attenuates canonical Wnt signaling via the Ror2 pathway."
    Development 137, 3293-3302 (2010).
  8. Eiraku, M, Watanabe, K., Matsuo-Takasaki, M., Kawada, M., Yonemura, S., Matsumura, M., Wataya, T., Nishiyama, A., Muguruma, K. and Sasai, Y.:
    "Self-Organized Formation of Polarized Cortical Tissues from ES cells and its Active Manipulation by Extrinsic Signals."
    Cell Stem Cell 3, 519-532 (2008).
  9. Wataya, T., Ando, S., Muguruma, K., Ikeda, H., Watanabe, K., Eiraku, M., Kawada, M., Takahashi, J., Hashimoto, N. and Sasai, Y.:
    "Minimization of Exogenous Signals in ES Cell Culture Induces Rostral Hypothalamic Differentiation."
    Proc Natl Acd Sci USA 105, 11796-11801. (2008).
  10. Inomata, H., Haraguchi, T. and Sasai, Y.:
    "Robust Stability of the Embryonic Dorsal Axial Pattern Requires ONT1, a Secreted Scaffold for Chordin Degradation."
    Cell 134, 854-865 (2008).

Members

Principal Investigator

Yoshiki SASAI
Group Director

Members

Hidehiko INOMATA
Senior Scientist
Keiko MUGURUMA
Research Specialist
Nozomu TAKATA
Research Scientist
Makoto NASU
Research Scientist
Hideya SAKAGUCHI
Technical Staff II
Ayaka NISHIYAMA
Technical Staff II
Masako KAWADA
Technical Staff II
Tomoko HARAGUCHI
Technical Staff II
Rieko YAKURA
Technical Staff II
Mika SOUEN
Technical Staff II