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Laboratory for Morphogenetic Signaling
Shigeo HAYASHI
Group Director
Shigeo HAYASHI (Ph.D.)
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Research Areas

Animal cells possess a great range of capabilities to sense environment, move, and differentiate to acquire specialized functions. Most of those autonomous functions are suppressed when a mass of cells give rise to a functional organ that consists of an array of differentiated cells with an ordered pattern. I am interested in uncovering signaling mechanisms that "socialize" cells to form a functional community during organogenesis. I will address this problem through analyses of two distinct types of organogenesis in Drosophila, development of appendages and the tracheal system. I will search for new gene functions involved in those processes and test their functions by the use of latest technologies of molecular imaging. 1. To understand the molecular basis for signaling systems regulating appendage development. Fundamental problems in appendage development are how cells in a two dimensional field of embryonic ectoderm interact to specify a new axis, proximal-distal, and how cell proliferation and differentiation are organized along this axis. We will search for genes involved in cell communication and cell sorting in appendage development by a large scale screening for gene functions. We will also address molecular mechanisms responsible for the specification of insect wings that share a common developmental origin as the leg but undergoes distinct developmental processes to acquire the flight function. 2. To understand the genetic basis for epithelial network formation A sheet of epithelium consisting of tightly connected cells is a basic unit for tubular organs. The tracheal system is a respiratory organ supplying the air directly into tissues by ramifying fine branches. Its formation is highly dynamic, driven by cell movement and cell rearrangement to match local demand for oxygen supply, while strictly maintaining epithelial integrity. We will address the problem of how tracheal cells respond to localized positional cues to construct highly ordered network of epithelia.

Research Subject

  1. Dynamics of epithelial architectures in morphogenesis
  2. Control of cytoskeletons in cell morphogenesis
  3. Mechanisms of insect limb development

Related links

  1. RIKEN Center for Developmental Biology Website_Laboratories PageNew Window
  2. Individual Website Laboratory PageNew Window

Press release

March 08, 2008
Cross purposes: Dual role for Src in control of cell-cell adhesionNew Window
November 16, 2007
Getting inside invaginationNew Window
August 17, 2006
Novel factor keeps cells in shape (Dr. Kenzi Oshima et al, CDB)New Window
June 16, 2006
Two nos make a yes: Double-negative regulation in Drosophila eye developmentNew Window

RIKEN RESEARCH

July 13, 2007
'Quality control' system for organogenesisNew Window
September 22,2006
Figuring out the shape of things
Two previously identified proteins play an unexpected role in regulating the formation and growth of a variety of important structures in the fruit fly New Window
July 28, 2006
Exposing the eye's 'turn-offs' and 'turn-ons'
A recent study has revealed an unusual 'double negative' regulatory system in fruit fly development and identified a key connection between two major signaling pathwaysNew Window

List of Selected Publications

  1. Otani, T., Oshima,K., Onishi, S., Takeda,M., Yonemura, S.,and Hayashi, S.:
    "IKKε regulates cell elongation through recycling endosome shuttling"
    Developmental Cell 20: 1-14(2011)
  2. Tajiri, R., Misaki, K., Yonemura, S., and Hayashi, S.:
    "Dynamic shape changes of ECM-producing cells drive morphogenesis of ball-and-socket joints in the fly leg"
    Development 137: 2055-2063(2010)
  3. Kawahashi, K., and Hayashi, S.:
    "Dynamic intracellular distribution of Notch during activation and asymmetric cell division revealed by functional fluorescent fusion proteins"
    Genes to Cells 15 :749-759(2010)
  4. Niwa, N., Akimoto-Kato, A., Niimi, T., Tojo, K., Machida, R., and Hayashi, S:
    "Evolutionary origin of the insect wing via integration of two developmental modules"
    Evolution & Development 12: 168-176(2010)
  5. Kaido,M., Wada,H., Shindo,M., and Hayashi,S.:
    "Essential requirement for RING finger E3 ubiquitin ligase Hakai in early embryonic development of Drosophila"
    Genes to Cells 14:1067-1077(2009)
  6. Kato,K., Hayashi,S.:
    "Practical guide of live imagining for developmental biologists"
    Develop. Growth Differ. 50:381-390 (2008)
  7. Kakihara,K., Shinmyozu,K., Kato,K., Wada,H., Hayashi,S.:
    "Conversion of plasma membrane topology during epithelial tube connection requires Arf-like 3 small GTPase in Drosophila"
    Mechanisms of Development , 125(3-4):325-336 (2008)
  8. Shindo,M., Wada,H., Kaido,M., Tateno,M., Aigaki,T., Tsuda,L., Hayashi,S.:
    "Dual function of Src in the maintenance of adherens junction during epithelial morphogenesis"
    Development 135(7):1355-64 (2008)
  9. Nishimura,M., Inoue,Y., Hayashi,S.:
    "A wave of EGFR signaling determines cell alignment and intercalation in the Drosophila tracheal placode"
    Development 134(23):4273-4282 (2007)
  10. Sakurai T,K.,Kojima,T., Aigaki,T.,Hayashi,S.:
    "Differential control of cell affinity required for progression and refinement of cell boundary during Drosophila leg segmentation."
    Developmental Biology 309:126-136 (2007)
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