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Mass Spectrometry Analysis Unit
Akira NAKAMURA
Unit Leader
Akira NAKAMURA (Ph.D.)
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Research Areas

The identification of the individual components of molecular complexes and elucidation of their structures can provide fundamental insights into the intricate mechanisms of development and regeneration. The Mass Spectrometry Analysis subunit uses LC-MASS spectrometry, a technology that enables the high-speed analysis of protein components from even minute biological samples. The subunit undertakes the analysis of protein modifications as well.

Related links

  1. RIKEN Center for Developmental Biology Website_Laboratories PageNew Window

List of Selected Publications

  1. Otani, T., Oshima, K., Onishi, S., Takeda, M., Shinmyozu, K., Yonemura, S. and Hayashi, S.:
    "IKKɛ Regulates Cell Elongation through Recycling Endosome Shuttling."
    Dev Cell. 20, 219-232. (2011)
  2. Hiragami-Hamada, K., Shinmyozu, K., Hamada, D., Tatsu, Y., Uegaki, K., Fujiwara, S. and Nakayama, J.:
    "N-Terminal Phosphorylation of HP1α Promotes Its Chromatin Binding."
    Mol Cell Biol. 31, 1186-1200. (2011)
  3. Shirai, A., Sadaie, M., Shinmyozu, K. and Nakayama, J.:
    "Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth."
    J Biol Chem. 285, 22448-22460. (2010)
  4. Hayakawa, T., Zhang, F., Hayakawa, N., Ohtani, Y., Shinmyozu, K., Nakayama, J. and Andreassen, P.R.:
    "MRG15 binds directly to PALB2 and stimulates homology-directed repair of chromosomal breaks."
    J Cell Sci. 123, 1124-1130. (2010)
  5. Okada, S., Nagabuchi, M., Takamura, Y., Nakagawa, T., Shinmyozu, K., Nakayama, J. and Tanaka, K.:
    "Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli: functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry."
    Plant Cell Physiol. 50, 1049-1061. (2009)
  6. Shimada, A., Dohke, K., Sadaie, M., Shinmyozu, K., Nakayama, J., Urano, T., and Murakami, Y.:
    "Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin."
    Genes Dev. 23, 18-23. (2009)
  7. Alev, C., McIntyre, B.A.S., Nagai, H. Shin, M., Shinmyozu, K., Jakt, L.M. and Sheng, G.:
    "BetaA, the major beta globin in definitive red blood cells, is present from the onset of primitive erythropoiesis in chicken"
    Developmental Dynamics,237, 1193-1197. (2008)
  8. Kakihara, K., Shinmyozu, K., Kato, K., Wada, H. and Hayashi, H.:
    "Conversion of plasma membrane topology during epithelial tube connection requires Arf-like 3 small GTPase in Drosophila."
    Mech. Dev.125, 325-336. (2008)
  9. Sadaie, M., Shinmyozu, K. and Nakayama, J.:
    "A conserved SET-domain methyltransferase, Set11, modifies ribosomal protein Rpl12 in fission yeast."
    J. Biol. Chem., 283, 7185-7195. (2008)
  10. Hayakawa, T., Ohtani, Y., Hayakawa, N., Shinmyozu, K., Saito, M., Ishikawa, F., and Nakayama, J.:
    "RBP2 is an MRG15 complex component and down-regulates intragenic histone H3 lysine 4 methylation."
    Genes Cells 12, 811-26. (2007)

Members

Principal Investigator

Akira NAKAMURA
Unit Leader

Members

Reiko NAKAGAWA
Research Specialist