Laboratory for Mammalian Molecular Embryology
Research Areas
| Anthony C.F. PERRY has worked on both molecular and cellular aspects of reproduction. Recent work has examined the components of spermatozoa that instruct the oocyte to commence the developmental program following sperm-egg fusion. The continued characterization of these components will permit a description of the signaling networks that become active in the early moments post-fertilization. Recent data from nuclear transfer cloning suggests that these early events have not only short-, but also long-term and subtle consequences for development. These analyses will utilize an integrated molecular, cellular and embryological approach out of which it is anticipated will also come opportunities to build on earlier work in the Perry laboratory on a novel method of genome manipulation known as metaphase II (mII) transgenesis. This method permits the efficient introduction of large DNA segments to produce transgenic mice, and promises additional routes to produce targeted knock-out and -down phenotypes, which the group is investigating. |  Team Leader Anthony C.F. PERRY (Ph.D.)  |
Research Subjects
| (1) | Networks in preimplantation embryos as predictors of development |
| (2) | Signaling and chromatin dynamics at fertilization |
| (3) | New transgenic methods of genome modulation |
List of Selected Publications
| (1) | Perry, A.C.F., and Verlhac, M.-H.: "Second meiotic arrest and exit in frogs and mice." EMBO Rep. 9, 246-251.(2008) |
| (2) | Yoshida, N., et al.: "Broad, ectopic expression of the sperm protein PLCZ1 induces parthenogenesis and ovarian tumours in mice." Development 134. 3941-52 (2007) |
| (3) | Yoshida, N., et al.: "Epigenetic discrimination by mouse metaphase II oocytes mediates asymmetric chromatin remodeling independently of meiotic exit." Dev Biol 301. 464-77 (2007) |
| (4) | Yoshida, N. and Perry, A.C.: "Piezo-actuated mouse intracytoplasmic sperm injection (ICSI)." Nat Protoc 2. 296-304 (2007) |
| (5) | Shoji, S., et al.: "Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20." Embo J 25. 834-45 (2006) |
| (6) | Amanai, M., et al.: "A restricted role for sperm-borne microRNAs in mammalian fertilization." Biol. Reprod. 75, 877-884.(2006) |
| (7) | Amanai, M., et al.: "Injection of mammalian metaphase II oocytes with short interfering RNAs to dissect meiotic and early mitotic events." Biol. Reprod. 75, 891-898.(2006) |
| (8) | Perry, A.C.F.: "Induced pluripotency and cellular alchemy." Nature Biotechnol. 24, 1363-1364. (2006). |
| (9) | Perry, A.C.F.: "Progress in human somatic-cell nuclear transfer." N. Engl. J. Med. 353, 87-88. (2005). |
| (10) | Perry, A.C.F.: "Nuclear transfer cloning and the United Nations." Nature Biotechnol. 22, 1506-1508. (2004). |