Laboratory for Stem Cell Biology
Research Areas
| Stem cells are one of the attractive sources for tissue regeneration. Recent technical advance to induce pluripotent stem cells from adult human somatic cells further prompted the research for guided differentiation of stem cells into specific cell lineages which can contribute to organ regeneration. Our research goal is to understand the mechanism of cell differentiation from pluripotent stem cells such as ES cells or iPS cells and the way how 'tissue -specific' stem cells are miantained as quiescent but expanded and recruited for tissue repair when necessary. We will study the in vitro differentiation of ES and iPS cells into mesodermal- hematopoietic or endothelial-cells and endodermal cells to utilize pluripotent stem cells for regeneration. As a model for tissue-specific stem cell system we will focus on melanocyte stem cell system especially aiming for the in vitro recaptulation of stem cell quinscence, proliferation and differentiation processes. |  Group Director Shin-Ichi NISHIKAWA (M. D., Ph. D.)  |
Research Subjects
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List of Selected Publications
| (1) | Eilken, H.M., Nishikawa, SI., and Schroeder, T.: "Continuous single-cell imaging of blood generation from haemogenic endothelium." Nature 457: 896-901 (2009) |
| (2) | Sasaki, Y., Calado, D.P, Derudder, E., Zhang, B., Shimizu, Y., Mackay, F., Nishikawa, SI., Rajewsky, K., Schmidt-Supprian, M.: "NIK overexpression amplifies, whereas ablation of its TRAF3-binding domain replaces BAFF: BAFF-R-mediated survival signals in B cells." PNAS 105: 10883-10888 (2008) |
| (3) | Era, T., Izumi, N., Hayashi, M., Tada, S., Nishikawa, S., and Nishikawa, SI.: "Multiple mesoderm subsets give rise to endothelial cells, ehwreas hematopoietic cells are differentiated only from a restricted subset in embryonic stem cell differentiation culture." Stem Cell 26: 401-411 (2008) |
| (4) | Yonetani, S., Moriyama, M., Nishigori, C., Osawa, M., and Nishikawa, SI.: "In vitro expansion of immature melanoblasts and their ability to repopulate melanocyte stem cells in the hair follicle." Journal of Investigative Dermatology vol.128, 408-420, (2008) |
| (5) | Takashima, Y., Era, T., Nakao, K., Kondo, S., Kasuga, M., Smith, A., and Nishikawa, SI.: "Neuroepithelial cells supply an initial transient wave of MSC differentiation." Cell 129:1377-1388 (2007) |
| (6) | Okuda, M., Togawa, A., Wada, H., and Nishikawa, SI.: "Distinct activities of stromal cells involved in the organogenesis of lymph nodes and peyer's patches." The Journal of Immunology 179:804-811 (2007) |
| (7) | Izumi, N., Era, T., Akimaru, H., Yasunaga, M., and Nishikawa, SI.: "Dissecting the molecular hierarchy for mesendoderm differentiation through a combination of embryonic stem cell culture and RNA interference." Stem Cell 25: 1664-1674 (2007) |
| (8) | Tanaka, Y., Era, T., Nishikawa, SI., and Kawamata, S.: "Forced expression of Nanog in hematopoietic stem cells results in a γδT-cell disorder." Blood 110 : 107-115 (2007) |
| (9) | Samokhvalov, I. M., Samokhvalova, N. I., and Nishikawa, S. I.: "Cell tracing shows the contribution of the yolk sac to adult haematopoiesis." Nature 446 (7139):1056-1061 (2007) |