Laboratory for Stem Cell Biology
Group Director
Shin-Ichi NISHIKAWA
(M.D., Ph.D.)
Stem cells are one of the attractive sources for tissue regeneration. Recent technical advance to induce pluripotent stem cells from adult human somatic cells further prompted the research for guided differentiation of stem cells into specific cell lineages which can contribute to organ regeneration. Our research goal is to understand the mechanism of cell differentiation from pluripotent stem cells such as ES cells or iPS cells and the way how 'tissue -specific' stem cells are miantained as quiescent but expanded and recruited for tissue repair when necessary. We will study the in vitro differentiation of ES and iPS cells into mesodermal- hematopoietic or endothelial-cells and endodermal cells to utilize pluripotent stem cells for regeneration. As a model for tissue-specific stem cell system we will focus on melanocyte stem cell system especially aiming for the in vitro recaptulation of stem cell quinscence, proliferation and differentiation processes.
- Guided differentiation of pluripotent stem cells into mesodermal and endodermal cells
- Factors required for the maintanance, proliferation and differentiation of stem cells
- Investigation of the epigenetic changes controlling differentiation, reprogramming and tumorgenesis
- Molecular mechanism of cell-cell interaction during lymphoid organogenesis
- February 13, 2009
- Endothelial cells give birth to blood
- June 30, 2007
- First MSC pathway revealed
- March 28, 2007
- Tracing bloodlines: Cell tagging shows an extraembryonic source of hematopoietic cells.
- September 29, 2006
- Neural crest gene ARID3B linked to childhood cancer malignancy
- February 01, 2006
- Tlx blazes a trail for blood vessel growth
- Kubota Yasushi, Osawa Masatake, Lars Jakt Martin, Yoshikawa Kazuaki, Nishikawa Shinichi:
"Necdin restricts proliferation of hematopoietic stem cells during hematopoietic regeneration"
Blood114:4383-4392(2009) - Kondo Nobuyuki, Ogawa Minetaro, Wada Hiromi, Nishikawa Shinichi:
"Thrombin induces rapid disassembly of claudin-5 from the tight junction of endothelial cells"
Experimental Cell Research315:2879-2887(2009) - Eilken H.M Nishikawa SI, Schroeder T:
"Continuous single-cell imaging of blood generation from haemogenic endothelium"
Nature 457: 896-901 (2009) - Sasaki Y, Calado D.P, Derudder E, Zhang B, Shimizu Y, Mackay F, Nishikawa SI, Rajewsky K, Schmidt-Supprian M:
"NIK overexpression amplifies, whereas ablation of its TRAF3-binding domain replaces BAFF: BAFF-R-mediated survival signals in B cells"
PNAS 105: 10883-10888 (2008) - Era T, Izumi N, Hayashi M, Tada S, Nishikawa S, Nishikawa SI:
"Multiple mesoderm subsets give rise to endothelial cells, ehwreas hematopoietic cells are differentiated only from a restricted subset in embryonic stem cell differentiation culture"
Stem Cell 26 : 401-411 (2008) - Yonetani S, Moriyama M, Nishigori C, Osawa M, Nishikawa SI:
"In vitro expansion of immature melanoblasts and their ability to repopulate melanocyte stem cells in the hair follicle."
Journal of Investigative Dermatology vol.128, 408-420, (2008) - Takashima Y, Era T, Nakao K, Kondo S, Kasuga M, Smith A, Nishikawa SI:.
"Neuroepithelial cells supply an initial transient wave of MSC differentiation."
Cell 129:1377-1388 (2007) - Okuda M, Togawa A, Wada H, Nishikawa SI:
"Distinct activities of stromal cells involved in the organogenesis of lymph nodes and peyer's patches."
The Journal of Immunology 179:804-811 (2007) - Izumi N, Era T, Akimaru H, Yasunaga M, Nishikawa SI:
"Dissecting the molecular hierarchy for mesendoderm differentiation through a combination of embryonic stem cell culture and RNA interference."
Stem Cell 25 : 1664-1674 (2007) - Tanaka Y, Era T, Nishikawa SI, Kawamata S:
"Forced expression of Nanog in hematopoietic stem cells results in a γδT-cell disorder ."
Blood 110 : 107-115 (2007)