Functional Genomics Unit
Unit Leader
Hiroki UEDA
(M.D., Ph.D.)
The Functional Genomics Unit has two missions. First, we provide functional genomics services to the thirty laboratories within the CDB. Second, we develop and introduce cutting-edge technologies related to functional genomics in order to accelerate the research that takes place in the CDB. To accomplish these two missions, we have been striving to implement two types of technologies, 1) expression analysis, and 2) high-throughput measurement and perturbation. For expression analysis, we first introduced GeneChip technology which is mainly used to measure the expression profiles of genes in cells or tissues. For high-throughput measurement and perturbation, we introduced cell-based screening technology which examines gene functions in cells. We will integrate the current expression analysis technology and the high-throughput measurement and perturbation technology to develop new functional genomics methods. In particular, we will focus on strengthening the single-cell expression analysis, as well as developing three-dimensional expression analysis of organs.
- Providing GeneChip service for CDB laboratories
- Development of single-cell expression analysis
- Development of organ-wide 3-dimensional expression analysis
- Kurimoto K, Yabuta Y, Ohinata Y, Ono Y, Uno KD, Yamada RG, Ueda HR, Saitou M.:
"An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis"
Nucleic Acids Res. vol. 34, no. 5, e42 (2006) - Matsumoto A, Ukai-Tadenuma M, Yamada RG, Houl J, Uno KD, Kasukawa T, Dauwalder B, Itoh TQ, Takahashi K, Ueda R, Hardin PE, Tanimura T, Ueda HR.:
"A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock"
Genes Dev. vol. 21, no. 13, 1687-1700 (2007) - Kawaguchi A, Ikawa T, Kasukawa T, Ueda HR, Kurimoto K, Saitou M and Matsuzaki F.:
"Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis"
Development, vol.135, no.18, 3113-3124 (2008) - Kumaki Y, Ukai-Tadenuma M, Uno KD, Nishio J, Masumoto K, Nagano M, Komori T, Shigeyoshi Y, Hogenesch JB and Ueda HR.:
"Analysis and synthesis of high-amplitude cis-elements in the mammalian circadian Clock"
Proc Natl Acad Sci U S A., vol.105, no.39, 14946-14951 (2008) - Isojima Y, Nakajima M, Ukai H, Fujishima H, Yamada RG, Masumoto KH, Kiuchi R, Ishida M, Ukai-Tadenuma M, Minami Y, Kito R, Nakao K, Kishimoto W, Yoo SH, Shimomura K, Takao T, Takano A, Kojima T, Nagai K, Sakaki Y, Takahashi JS, Ueda HR.:
"CKIepsilon/delta-dependent phosphorylation is a temperature-insensitive, period-determining process in the mammalian circadian clock"
Proc Natl Acad Sci U S A. vol. 106, no. 37, 15744-15749 (2009) - Alev C, Wu Y, Kasukawa T, Jakt LM, Ueda HR, Sheng G.:
"Transcriptomic landscape of the primitive streak, Development"
Development, vol.137, no.17, 2863-2874 (2010) - Masumoto K, Ukai-Tadenuma M, Kasukawa T, Nagano M, Uno KD, Tsujino K, Horikawa K, Shigeyoshi Y, Ueda HR.:
"Acute induction of Eya3 by late-night light stimulation triggers TSHβ expression in photoperiodism"
Curr Biol. vol.20, no.24, 2199-2206 (2010)
Principal Investigator
- Hiroki UEDA
- Unit Leader
Members
- Itoshi NIKAIDO
- Research Specialist
- Takeya KASUKAWA
- Research Specialist
- Youhei SASAGAWA
- Special Postdoctoral Researcher
- Hiroshi FUJISHIMA
- Technical Staff I
- Kenichirou UNO
- Technical Staff I
- Junko SAKAI
- Technical Staff II