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Genetic Engineering Unit
Shinichi AIZAWA
Unit Leader
Shinichi AIZAWA (Ph.D.)
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Research Areas

Quality and efficacy of the research are greatly dependent on how easily mutant mice are handled. The major aim of this laboratory is to develop mutant mice useful for researches in Developmental Biology and Regenerative Medicinebyin collaboration with researchers not only in CDB but also in Japan and Asian. With the sequence information on genes from researchers, the construction of vectors, ES culture, isolation of homologous recombinants, production of chimera as well as generation of transgenic miceare all being done speedily by this laboratory. Special focus is in development of mutant mice, embry oculture and microscopic techniques for bioimaging in mouse embryos.

Research Subject

  1. Generation of mouse mutants useful for developmental biology and regenerative medicine
  2. Development of mutant mice and techniques for bioimaging in mouse embryos
  3. Development of technology to manipulate mouse embryos genetically and embryologically

Related links

  1. RIKEN Center for Developmental Biology Website_Laboratories PageNew Window

RIKEN RESEARCH

July 13,2007
Heading in the right direction
Researchers find a gene controlling embryo orientation New Window

List of Selected Publications

  1. Kiyonari, H., Kaneko, M., Abe, S. and Aizawa, S.:
    "Three Inhibitors of FGF Receptor, ERK, and GSK3 Establishies Germline-Competent Embryonic Stem Cells of C57BL/6N Mouse Strain With High Efficiency and Stability."
    Genesis. 48, 317-327(2010).
  2. Yamanishi Y, Kitaura J, Izaawa K, Kitani A, Komeno Y, Nakamra M, Yamazaki S, Enomoto Y, Oki T, Akiba H, Abe T, Komori T, Morikawa Y, Kiyonari H, Takai T, Okumura K, Kitamura T.:
    "TIM1 is an endogenous ligand for LMIR5/CD300b:LMIR5 deficiency ameliorates mouse kidney ischemia/reperfusion injury."
    JEM 207, 1501-1511(2010).
  3. Uchiyama, Y., Skaguchi, M., Terabayashi, T., Inenaga, T., Inoue, S., Kobayashi, C., Oshima, N., Kiyonari, H., Nakagata, N., Sato, Y., Sekiguchi, K., Miki, H., Araki, E., Fujimura, S., Tanaka, S, S., Nishinakamura, R.:
    "Kif26b, a kinesin family gene, regulates adhesion of the embryonic kindney mesenchyme."
    PNAS. 107, 9240-9245(2010).
  4. Oboki K, Ohno T, Kajiwara N, Arae K, Morita H, Ishii A, Nambu A, Abe T, Kiyonari H, Matsumoto K, Sudo K, Okumura K, Saito H, Nakae S.:
    "IL-33 is a crucial amplifier of innate rather than acquired immunity."
    Proc Natl Acad Sci U S A. 107(43), 18581-18586(2010).
  5. Tao H, Manak R, Sowers L, Mei X, Kiyonari H, Abe T, Dahdaleh NS, Yang T, Wu S, Chen S. Fox MH, Gurnett C. Montine T, Bird T, Shaffer LG, Rosenfeld JA, McConnell J, Madan-Khetarpal S, Berry-Kravis E, Griesbach H, Saneto R, Scott MP, Antic D, Reed J, Boland,:
    "Mutations in Prickle Orthologs Cause Seizures in Flies, Mice, and Humans."
    American Journal of Human Genetics 88, 1-12(2011).
  6. Kobayashi M, Sabouri Z, Sabouri S, Kitawaki Y, Pommier Y, Abe T, Kiyonari H and Honjo T.:
    "Decrease in topoisomerase I is responsible for activation-induced cytidine deaminase (AID)-dependent somatic hypermutation."
    Proc. Natl. Acad. Sci. USA. 108(48)., 19305-19310(2011)
  7. Sato Y, Iketani M, Kurihara Y, Yamaguchi M, Yamashita N, Nakamura F, Arie Y, Kawasaki T, Hirata T, Abe T, Kiyonari H, Strittmatter SM, Goshima Y, Takei K.:
    "Cartilage acidic protein-1B (LOTUS), an endogenous Nogo receptor antagonist for axon tract formation."
    Science.333(6043)., 769-773(2011)
  8. Shioi G, Kiyonari H, Abe T, Nakao K, Fujimori T, Jang CW, Huang CC, Akiyama H, Behringer RR, Aizawa S.:
    "A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging."
    Genesis. 49(7): 570-578(2011)
  9. Abe T, Kiyonari H, Shioi G, Inoue K, Nakao K, Aizawa S, Fujimori T.:
    "Establishment of conditional reporter mouse lines at ROSA26 locus for live cell imaging."
    Genesis. 49(7): 579-90(2011)
  10. Zhang J, Eto K, Honmyou A, Nakao K, Kiyonari H, Abé S.:
    "Neuregulins are essential for spermatogonial proliferation and meiotic initiation in neonatal mouse testis."
    Development. 138(15):3159-3168(2011)

Members

Principal Investigator

Shinichi AIZAWA
Unit Leader

Members

Go SHIOI
Research Specialist
Hiroshi KIYONARI
Research Specialist
Kenji OKAZAKI
Research Scientist
Kana KADOWAKI
Technical Staff I
Takaya ABE
Technical Staff I
Yoshiko MUKUMOTO
Technical Staff I
Naoko HATAMOTO
Technical Staff II
Mari KANEKO
Technical Staff II
Michiko HIGASHIKAWA
Technical Staff II
Megumi WATASE
Technical Staff II