Bioresource Engineering Division
To support the projects undertaken by the Department of Biological Systems, we develop new techniques for practical genetics, which are essential for consistent, high quality maintenance and supplies of bioresources. The techniques which are to be developed immediately include those related to nuclear transfer, establishment of stem cell lines of various origins, and cryopreservation of embryos/gametes.
- Establishment of methods for cryopreservation of embryos and germ cells
- Establishment of methods for microinsemination (ICSI)
- Establishment of methods for nuclear transfer cloning
- Establishment of new stem cell lines for research use
- September 17,2010
- Near tenfold improvement achieved in mouse somatic cell clone birthrate
- August 6,2010
- First protocol for the production of rabbit-cultured iPS cells
- March 31, 2009
- Success in doubling of mouse growth through use of an immature embryonic cell, making possible generational change in forty four days, half of the established rate and the shortest rate among vertebrates
- July 17, 2007
- Gene Engineering Division of BRC, in association with Kyoto University, succeeded in the world's first separation and culturing of capsular stem cells which generate sex hormones in the mouse ovary.
- Matoba S, Inoue K, Kohda K, Sugimioto M, Mizutani E, Ogonuki N, Nakamura T, Abe K, Nakano T, Ishino F, and Ogura A.:
"RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos. "
Proc Natl Acad Sci USA, 108, 20621-20626 (2011) - Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A.:
"Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer."
Science, 330, 496-499 (2010) - Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A.:
"Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells."
Proc Natl Acad Sci USA, 104, 12389-12394 (2007) - Ogonuki, N., Mochida, K., Miki, H., Inoue, K., Fray, M., Iwaki, T., Moriwaki, K., Obata, Y., Morozumi, K., Yanagimachi, R., and Ogura A.:
"Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring"
Proc. Natl. Acad. Sci. USA, 103, 13098-13103 (2006) - Inoue, K., Ogonuki, N., Miki, H., Hirose, M., Noda, S., Kim, J-M., Aoki, F., Miyoshi, H,, and Ogura A.:
"Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer"
J. Cell Sci. 119, 1985-1991(2006) - Inoue, K., Wakao, H., Ogonuki, N., Miki, H., Seino, K., Nambu-Wakao, R., Noda, S., Miyoshi, H., Koseki, H., Taniguchi, M., and Ogura, A.:
"Generation of cloned mice by direct nuclear transfer from natural killer T cells"
Curr. Biol. 15, 1114-1118 (2005) - Kanatsu-Shinohara, M., Inoue, K., Lee, J., Yoshimoto, M., Ogonuki, N., Miki, H., Baba, S., Kato, T., Kazuki, Y., Toyokuni, S., Oshimura, M., Heike, T., Nakahata, T., Ishino, F., Ogura, A., and Shinohara, T.:
"Generation of pluripotent stem cells from neonatal mouse testis"
Cell 119, 1001-1012 (2004) - Fulka, J Jr., Miyashita, N., Nagai, T., and Ogura, A.:
"Do cloned mammals skip a reprogramming step"
Nat. Biotechnol. 22, 25-26 (2004) - Ogonuki, N., Inoue, K., Yamamoto, Y., Noguchi, Y., Tanemura, K., Suzuki, O., Nakayama, H., Doi, K., Ohtomo, K., Satoh, M., Nishida, A., and Ogura, A.:
"Early death of mice cloned from somatic cells"
Nat. Genet. 30, 253-254 (2002) - Inoue, K., Kohda, T., Lee, J., Ogonuki, N., Mochida, K., Noguchi, Y., Tanemura, K., Kaneko-Ishino, T., Ishino, F., and Ogura, A.:
"Faithful expression of imprinted genes in cloned mice"
Science 295, 297 (2002)
Principal Investigator
- Atsuo OGURA
- Head
Staff Scientist
- Kimiko INOUE
- Senior Research Scientist
- Keiji MOCHIDA
- Senior Technical Scientist
- Narumi OGONUKI
- Senior Technical Scientist
Postdoctoral Fellow
- Shogo MATOBA
- Special Postdoctoral Researcher
- Eiji MIZUTANI
- Contract Researcher
Technical Assistant
- Michiko HIROSE
- Technical Staff II
- Ayumi HASEGAWA
- Technical Staff II

