Live Cell Molecular Imaging Research Team
Team Leader
Akihiko NAKANO (D.Sci.)
Imaging is one of the most important methodologies of the modern life science. Development of useful fluorescent/luminescence probes and the advancement of light microscope technologies enable us real-time observation of living cells and tissues. We will exploit cutting-edge microscopic technologies with visible light, and observe, measure and manipulate proteins and membranes within living cells to understand mechanisms of live phenomena. We will also aim at introducing the new light sources that are created by other teams of the Extreme Photonics Department into the imaging technologies of live materials.
- cutting-edge live imaging with visible light
- single-molecule visualization and measurement
- imaging with new light sources
- Funakoshi, T., Clever, M., Watanabe, A., Imamoto, N.:
"Localization of Pom121 to the inner nuclear membrane is required for an early step of interphase NPC assembly"
Mol. Biol. Cell. February 2 (2011) [Epub ahead of print] - Yasuhito Maeda, Masaki Yumoto, Norito Saito, Takayo Ogawa, Kurokawa. Kurokawa, Akihiko Nakano, Msafumi Yamashita and Satoshi Wada.:
"Broadly tunable UV-blue picosecond pulsed laser andiIts application for biological imaging"
Optical Review 17, 305-308 (2010) - Maeshima K, Iino H, Hihara S, Funakoshi T, Watanabe A, Nishimura M, Nakatomi R, Yahata K, Imamoto F, Hashikawa T, Yokota H, Imamoto N.:
"Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase."
Nat Struct Mol Biol. 17, 1065-1071. (2010) - Masakazu Iwai, Kenji Takizawa, Ryutaro Tokutsu, Akira Okamuro, Yuichiro Takahashi, and Jun Minagawa.:
"Isolation of the elusive supercomplex that drives cyclic electron flow in photosynthesis"
Nature 464:1210-1213 (2010). - Murate M, Hayakawa T, Ishii K, Inadome H, Greimel P, Watanabe Y, Nagatsuka Y, Ito K, Ito Y, Takahashi H, Hirabayashi Y, Kobayashi T.:
"Phosphatidylglucoside forms specific lipid domains on the outer leaflet of the plasma membrane"
Biochemistry 49: 4732-4739 (2010). - Michio Hiroshima and Yasushi Sako.:
"Single-molecule kinetic analysis of receptor protein tyrosine kinases"
Cell Signaling Reactions: Single-molecule Kinetic Analyses", pp.1-32. Sako, Y. and Ueda, M. eds. Springer, 2010. - Tahara, K,, Takagi, M., Ohsugi, M., Sone,T., Maeshima, K., Horiuchi,Y., Tokai-Nishizumi, N., Fumiko Nishiumi, F., Imamoto, F., Yamamoto, T., Kose, S. and Imamoto, N.:
"Importin β and small GTPase Ran mediates the chromosome loading of human chromokinesin KID."
J. Cell Biol, 180, 493-506 (2008) - Takahashi, M., Murate, M., Fukuda, M., Sato, S. B., Ohta, A., and Kobayashi, T.:
"Cholesterol controls lipid endocytosis through rab11"
Mol. Biol. Cell 18, 2667-2677 (2007) - Iwamoto, K., Hayakawa, T., Murate, M., Makino, A., Ito, K., Fujisawa, T., and Kobayashi, T.:
"Curvature-dependent recognition of ethanolamine phospholipids by duramycin and cinnamycin"
Biophys. J., 93, 1608-1619 (2007) - Matsuura-Tokita, K., Takeuchi, M., Ichihara, A., Mikuriya, K. and Nakano, A.:
"Live imaging of yeast Golgi cisternal maturation"
Nature 441, 1007-1010 (2006)