Chemical Library Validation Team
Team Head
Nobumoto WATANABE (D.Sci.)
The Chemical Biology Core Facility collects many natural compounds and stores them in the RIKEN Natural Product Depository (NPDepo). A good chemical library should contain many compounds with various useful biological activities, therefore our team will validate the activity of the compounds in NPDepo by actual screening of the library through various assay systems. For this purpose, we will develop high throughput screening systems using chemical array and liquid handling robots to screen the large library. Besides developing new screening systems to identify compounds with novel biological activities, our team also conducts basic research to study the mechanisms behind the biological activities of the compounds.
- Construction of the screening system for small molecules with novel molecular targets
- Validation of NPDepo through various screening systems
- Analysis of the mechanisms behind the activities of compounds with novel biological activity
- Chemical Biology on the molecular targets of bioprobes
- Mining and functional analysis of novel molecular targets
- I. Miyazaki, S. Simizu, H. Okumura, S. Takagi, H. Osada:
"A small-molecule inhibitor shows that pirin regulates migration of melanoma cells."
Nat. Chem. Biol. 6, 667-673 (2010) - M. Muroi, S. Kazami, K. Noda, H. Kondo, H. Takayama, M. Kawatani, T. Usui, H. Osada:
"Application of proteomic profiling based on 2D-DIGE for classification of compounds according to the mechanism of action."
Chem Biol., 17, 460-470 (2010) - N. Watanabe, T. Sekine, M. Takagi, J. I.. Iwasaki, N. Imamoto, H. Kawasaki, H. Osada:
"Deficiency in chromosome congression by the inhibition of PLK1 polo box domain-dependent recognition."
J. Biol. Chem., 284, 2344-2353 (2009) - Y. Tamura, S. Simizu, M. Muroi, S. Takagi, M. Kawatani, N. Watanabe, H. Osada:
"Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis."
Oncogene, 28, 107-116 (2009) - M. Kawatani, H. Okumura, K. Honda, N. Kanoh, M. Muroi, N. Dohmae, M. Takami, M. Kitagawa, Y. Futamura, M. Imoto, H. Osada:
"The identification of an osteoclastogenesis inhibitor through the inhibition of glyoxalase I.."
Proc. Natl. Acad. Sci. USA., 105, 11691-11696 (2008)