Chemical Library Validation Team
Team Head
Nobumoto WATANABE (D.Sci.)
The Chemical Biology Core Facility collects many natural compounds and stores them in the RIKEN Natural Product Depository (NPDepo). A good chemical library should contain many compounds with various useful biological activities, therefore our team will validate the activity of the compounds in NPDepo by actual screening of the library through various assay systems. For this purpose, we will develop high throughput screening systems using chemical array and liquid handling robots to screen the large library. Besides developing new screening systems to identify compounds with novel biological activities, our team also conducts basic research to study the mechanisms behind the biological activities of the compounds.
- Construction of the screening system for small molecules with novel molecular targets
- Validation of NPDepo through various screening systems
- Analysis of the mechanisms behind the activities of compounds with novel biological activity
- Chemical Biology on the molecular targets of bioprobes
- Mining and functional analysis of novel molecular targets
- E.B. Ong, N. Watanabe, A. Saito, Y. Futamura, K.H. Abd El Galil, A. Koito, N. Najimudin, H. Osada,:
"Vipirinin, a coumarin-based HIV-1 Vpr Inhibitor, interacts with a hydrophobic region of Vpr."
J. Biol. Chem. 286, 14049-14056 (2011) - M. Kawatani, H. Takayama, M. Muroi, S. Kimura, T. Maekawa, H. Osada,:
"Identification of a small-molecule inhibitor of DNA topoisomerase II by proteomic profiling."
Chem. Biol. 18, 743-751 (2011) - I. Miyazaki, S. Simizu, H. Okumura, S. Takagi, H. Osada,:
"A small-molecule inhibitor shows that pirin regulates migration of melanoma cells."
Nat. Chem. Biol. 6, 667-673 (2010) - M. Muroi, S. Kazami, K. Noda, H. Kondo, H. Takayama, M. Kawatani, T. Usui, H. Osada,:
"Application of proteomic profiling based on 2D-DIGE for classification of compounds according to the mechanism of action."
Chem Biol., 17, 460-470 (2010) - N. Watanabe, T. Sekine, M. Takagi, J. I.. Iwasaki, N. Imamoto, H. Kawasaki, H. Osada,:
"Deficiency in chromosome congression by the inhibition of PLK1 polo box domain-dependent recognition."
J. Biol. Chem., 284, 2344-2353 (2009) - Y. Tamura, S. Simizu, M. Muroi, S. Takagi, M. Kawatani, N. Watanabe, H. Osada,:
"Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis."
Oncogene, 28, 107-116 (2009)
Principal Investigator
- Nobumoto WATANABE
- Team Head
Staff Scientist
- Tatsuhiko SUDO
- Senior Research Scientist
- Makoto MUROI
- Senior Research Scientist
- Takuya ITO
- Senior Research Scientist
- Yasumitsu KONDOH
- Senior Research Scientist
- Akira WADA
- Senior Research Scientist
- Makoto KAWATANI
- Senior Research Scientist
Postdoctoral Fellow
- Suresh PANTEE
- Foreign Postdoctoral Researcherr
- Hiroki HAYASE
- Postdoctoral Researcher
- Tatsuro KAWAMURA
- Postdoctoral Researcher
- Alena LAGUMDZIJA
- Visiting Researcher
Technical Assistant
- Hideaki KONNO
- Technical Staff I
- Kaori HONDA
- Technical Staff I
- Hisae KONDO
- Technical Staff I
- Yasuko HIRATA
- Technical Staff I
- Harumi AONO
- Technical Staff I
- Emiko SANADA
- Technical Staff I
- Yukako FUKUSHIMA
- Technical Staff I
- Shinobu YAMAGUCHI
- Technical Staff I
- Tomomi SEKINE
- Technical Staff I
Visiting Research Staff
- Osong KWON
- Visiting Scientist