Chemical Genomics Research Group
Group Dirctor
Minoru YOSHIDA (D.Agr.)
Chemical biology using chemistry as leverage has unraveled complex biological functions and has contributed much to the discovery of novel phenomena and their underlying mechanisms. Thanks to the recent technical advances, chemical biology has been able to develop more comprehensive approach to "Chemical Genomics". This project focuses on the development of a variety of original assay systems using newly identified genes, proteins and cell lines, and on the discovery of unique compounds (molecular ligands) that target specific biological molecules. Based on the activity of such novel molecular ligands, our effort will then be directed toward the identification of their target molecules and the elucidation of the role of their biological functions. Our goal is to deeply understand the biological systems and to construct the bases of drug discovery.
- Construction of unique assay systems and screening for molecular ligands
- Design and synthesis of novel of molecular ligands and development of labeled probes
- Target identification of molecular ligands
- Mode of action of molecular ligands and its application to life sciences
- June 14, 2010
- New research uncovers elusive target of antifungal agent
- Nishimura, S., Arita, Y., Honda, M., Iwamoto, K., Matsuyama, A., Shirai, A., Kawasaki, H., Kakeya, H., Kobayashi, T., Matsunaga, S., and Yoshida, M.:
"Marine antifungal theonellamides target 3beta-hydroxysterol to activate Rho1 signaling."
Nat. Chem. Biol., 6: 519-526, 2010. - Maekawa, T., Jin, W., and Ishii, S.:
"The role of ATF-2 family transcription factors in adipocyte differentiation: antiobesity effects of p38 inhibitors."
Mol. Cell. Biol., 30: 613-625, 2010. - Megumi Ohkubo, Go Hirai, Mikiko Sodeoka.:
"Synthesis of the DFGH ring system of Type B Physalins: Highly Oxygenated, Cage-Shaped Molecules"
Angew. Chem. Int. Ed. 48, 3862-3866, 2009. - Sasaki, K., Ito, T., Nishino, N., Khochbin, S., and Yoshida, M.:
"Real-time imaging of histone H4 hyperacetylation in living cells."
Proc. Natl. Acad. Sci. U.S.A., 106: 16257-16262, 2009. - Ho, C. H., Magtanong, L., Barker, S. L., Gresham, D., Nishimura, S., Natarajan, P., Koh, J. L. Y., Proter, J., Gray, C. A., Andersen, R. J., Giaever, G., Nislow, C., Andrews, B., Botstein, D., Graham, T. R., Yoshida, M., and Boone, C.:
"A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds."
Nature Biotechnol., 27: 369-377, 2009. - Taisuke Watanabe, Kiichiro Totani, Ichiro Matsuo, Jun-ichi Maruyama, Katsuhiko Kitamoto, and Yukishige Ito.:
"Genetic analysis of glucosidase II β-subunit in trimming of high-mannose-type glycans."
Glycobiology 19, 834-840, 2009. - Fukuda, I., Ito, A., Uramoto, M., Saitoh, H., Kawasaki, H., Osada, H., and Yoshida, M.:
"Kerriamycin B inhibits protein SUMOylation."
J. Antibiotics, 62: 221-224, 2009. - Tatsukawa, H., Fukaya, Y., Frampton, G., Martinez-Fuentes, A., Suzuki, K., Kuo, T.-F., Nagatsuma, K., Shimokado, K., Okuno, M., Wu, J., lismaa, S., Matsuura, T., Tsukamoto, H., Zern, M. A., Graham, R. M., and Kojima, S.:
"Role of transglutaminase 2 in liver injury via crosslinking and silencing of transcription factor, Sp1."
Gastroenterology, 136: 1783-1795, 2009. - Fukuda, I., Ito, A., Hirai, G., Nishimura, S., Kawasaki, H., Saitoh, H., Kimura, K., Sodeoka, M., and Yoshida, M.:
"Ginkgolic acid inhibits protein SUMOylation by blocking formation of the E1-SUMO intermediate."
Chem. Biol., 16: 133-140, 2009. - Yashiroda, Y., Matsuyama, A., and Yoshida, M.:
"New insights into chemical biology from ORFeome libraries."
Curr. Opin. Chem. Biol., 12: 55-59, 2008.
Principal Investigator
- Minoru YOSHIDA
- Group Dirctor
Staff Scientist
- Tetsuo ONUKI
- Akiko MASUDA
- ASI Technical Scientist
Postdoctoral Fellow
- Yasushi TAKEMOTO
- Visiting Researcher
| Molecular Ligand Discovery Research Team |
| Molecular Ligand Synthesis Research Team |
| Molecular Ligand Target Research Team |
| Molecular Ligand Biology Research Team |