Cheminformatics and Compound Creation Team
Team Head
Shunji TAKAHASHI (D.Sci.)
We systematically isolate secondary metabolites from microorganisms and derivatize natural products by genetic engineering. We develop Natural products (NP) plot database to efficiently isolate metabolites with various structures. We are trying to diversify valuable metabolites by modifying gene clusters in the microorganisms. In this project, we utilize Streptomyces genome sequence information and fungal genome sequence database. The structural diversity of secondary metabolite is also increased by chemical modification. Structure-activity relationship studies are approaches used for chemical optimizations.
- Systematic construction of secondary microbial metabolite library
- Production of various new chemicals using genetic engineering methods
- Target oriented chemical modification and synthesis of bioprobes
- Construction of chemical database
- S. Takahashi, A. Toyoda, Y. Sekiyama, H. Takagi, T. Nogawa, M. Uramoto, R. Suzuki, H. Koshino, T. Kumano, S. Panthee, T. Dairi, J. Ishikawa, H. Ikeda, Y. Sakaki, and H. Osada,:
"Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation. "
Nat. Chem. Biol., 7, 461-468 (2011) - N. Kato, M. Tokuoka, Y. Shinohara, M. Kawatani, M. Uramoto, Y. Seshime, I. Fujii, K. Kitamoto, T. Takahashi, S. Takahashi, Y. Koyama, and H. Osada,:
"Genetic safeguard against mycotoxin cyclopiazonic acid production in Aspergillus oryzae."
ChemBioChem, 12, 1376-1382 (2011) - N. Kato, H. Suzuki, H. Takagi, M. Uramoto, S. Takahashi, and H. Osada,:
"Gene disruption and biochemical characterization of verruculogen synthase of Aspergillus fumigates. "
ChemBioChem, 12, 711-714 (2011) - Y. Sun, F. Hahn, Y. Demydchuk, J. Chettle, M. Tosin, H. Osada, and P.F. Leadlay,:
"In vitro reconstruction of tetronate RK-682 biosynthesis."
Nat. Chem. Biol., 6, 99-101 (2010) - S. Takahashi, H. Takagi, A. Toyoda, M. Uramoto, T. Nogawa, M. Ueki, Y. Sakaki, and H. Osada,:
"Biochemical characterization of a novel indole prenyltransferase from Streptomyces sp. SN-593."
J. Bacteriol., 192, 2839-2851 (2010) - T. Nogawa, A. Okano, S. Takahashi, M. Uramoto, H. Konno, T. Saito, and H. Osada,:
"Verticilactam, a new macrolactam isolated from a microbial metabolite fraction library."
Org. Lett., 12, 4564-4567 (2010) - N. Kato, H. Suzuki, H. Takagi, Y. Asami, H. Kakeya, M. Uramoto, T. Usui, S. Takahashi, Y. Sugimoto, and H. Osada,:
"Identification of cytochrome P450s required for fumitremorgin biosynthesis in aspergillus fumigatus."
ChemBioChem, 10, 920-928 (2009)
Principal Investigator
- Shunji TAKAHASHI
- Team Head
Staff Scientist
- Takayuki MOTOYAMA
- Senior Research Scientist
- Masashi UEKI
- Senior Research Scientist
- Masae HORINOUCHI
- Senior Research Scientist
- Naoki KATO
- ASI Research Scientist
- Toshihiko NOGAWA
- ASI Technical Scientist
Postdoctoral Fellow
- Choong Soo YUN
- Contract Researcher
- Yushi FUTAMURA
- Postdoctoral Researcher
- Ken-ichi TANAKA
- Postdoctoral Researcher
Technical Assistant
- Hiroshi TAKAGI
- Technical Staff I
- Akiko OKANO
- Technical Staff I
- Eri OOWADA
- Technical Staff I
- Kumi SUGINO
- Technical Staff I
- Mizue YUKI
- Technical Staff I
- Naoko MORITA
- Technical Staff II