Analysis Protocol Team

In collaboration with the Rigaku corporation, we propose comprehensive protocols for protein research through utilizing the structure analysis platform which was constructed in the Protein 3000 Project and was enhanced by the RIKEN SPring-8 Center. And the RIKEN's technologies for protein analysis will be put into practical use in general bio-science laboratories.

Research Subjects

• Making protocols for protein research
• Application of protein analysis technologies to practical use

Publications

1. Asada Y., Sugahara M., Mizutani H., Naitow H., Tanaka T., Matsuura Y., Agari Y., Ebihara A., Shinkai A., Kuramitsu S., Yokoyama S., Kaminuma E., Kobayashi N., Nishikata K., Shimoyama S., Toyoda T., Ishikawa T., and Kunishima N.:
   "Integrated database of information from structural genomics experiments"
   Acta Crystallogr. D, in press.
2. Sugahara M.:
   "A fiber-based crystal mounting technique for protein cryocrystallography"
   J. Appl. Cryst. 45, 362-366 (2012)
3. Matsuura Y, Takehira M, Sawano M, Ogasahara K, Tanaka T, Yamamoto H, Kunishima N, Kato E, and Yutani K.:
   "Role of charged residues in stabilization of Pyrococcus horikoshii CutA1, which has a denaturation temperature of nearly 150 °C"
   FEBS J. 279, 78-90 (2012)
4. Sugahara M., Kageyama-Morikawa Y., and Kunishima N.:
   "Packing space expansion of protein crystallization screening with synthetic zeolite as a heteroepitaxic nucleant"
   Crystal Growth & Design 11, 110-120 (2011)