Laboratory for Nano-Bio Probes

Our research team develops novel nano-bio probes to visualize molecular dynamics and cellular dynamics from in vitro to in vivo for understanding of the dynamic biological systems. We synthesize fluorescent nanoprobes for multiplexed single molecular imaging, functional nanoprobes for controlling cellular state by using light and magnetic field, and highly fluorescent near-infrared probes for investigating cellular dynamics in living tissues. In a whole body system, visible light cannot pass through tissues owing to the strong absorption by intrinsic chromophores such as hemoglobin, while near-infrared light (700-1500 nm) shows high permeability for the tissues due to their low absorption and scattering. Our team develops a novel imaging technique for cellular dynamics in deep tissues at the near-infrared region from 1000-1500 nm, which called as "2nd optical window for biological system". For this imaging technique, our team will develop a probe delivery technique for targeting specific cells, tissues and organs.

Research Subjects

• Highly fluorescent probes for single molecule imaging in living cells.
• Functional nanoprobes for controlling cellular states by using light and magnetic filed.
• Highly fluorescent near-infrared probes for deep tissue imaging
• Probe delively techniques for targetting cells, tissues, and organs
• In vivo near infrared fluorescence imaging of cellular dynamics in living systems

Publications

1. Hasegawa M., Tsukasaki Yoshikazu T., Tatsuya Ohyanagi T., Jin T.:
   "Bioluminescence resonance energy transfer coupled near-infrared QDs using GST-tagged luciferase for in vivo imaging"
   Chem. Commun. 49, 228-230 (2013)
2. Ma Q., Nakane Y., Mori Y., Hasegawa M.,Yoshioka Y., Watanabe T. M., Gonda K., Ohuchi N., Jin T.:
   "Multilayered, Core/shell Nanoprobes Based on Magnetic Ferric Oxide Particles and Quantum Dots for Multimodality Imaging of Breast Cancer Tumors"
   Biomaterilas, 33, 8486-8494, (2012)
3. Nakane Y., Sasaki A., Kinjo M., Jin T.:
   "Bovine serum albumin-coated quantum dots as a cytoplasmic viscosity probe in a single living cell"
   Anal. Methods, 4 (7), 1903 -1905 (2012)
4. Ohmachi M., Komori Y., Iwane A. H., Fujii F., T. Jin T., Yanagida T.:
   "Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod-tagged myosin V"
   Proc. Natl. Acad. Sci. USA., 109, 5294-5298 (2012)
5. Tiawari D. K., Jin T., Behari J.:
   "Bio-distribution and toxicity assessment of intravenously injected anti-HER2 antibody conjugated CdSe quantum dots in Wistar rat"
   Int. J. Nanomed. 6, 463-475 (2011)
6. Tanaka S.-I., Miyazaki J., Tiawari D. K., Jin T., Inouye Y.:
   "Fluorescent platinium (Pt5) nanoclusters: their synthesis, purification, characterization, and application to bio-imaging."
   Angew. Chem. Int. Ed., 50, 431-435 (2011)
7. Tatsuya T., Nagahori N., Shimawaki K., Yamashita T., Hinou H., Sasaki A., Jin T., Kinjo M., Nishimura S. I.:
   "Importance of sialic acid residues illuminated by live animal imaging using phosphorylcholine self-assembled monolayers-coated quantum dots"
   J. Am. Chem. Soc.133, 12507-12517, (2011)
8. Jin T., Tiwari D. K., Tanaka S.-I., Inouye Y., Yoshizawa K.,and Watanabe T. M.:
   "Antibody-ProteinA conjugated quantum dots for multiplexed imaging of surface receptors in living cells"
   Molecular. BioSystems, 6. 2325-2332 (2010)
9. Watanabe T. M., Fukui S., Jin T., Fujii F., Yanagida T.:
   "Real-time nanoscopy by using blinking enhanced quantum dots"
   Biophys. J. 99, L50-L52 (2010)
10. Jin T., Sasaki A., Kinjo M., Miyazaki J.:
    "A quantum dot-based ratiometric pH sensor"
    Chem. Commun., 46, 2408-2410 (2010)