Laboratory for Integrative Genomics

The very basic mission of integrative genomics group is to function as a "Gateway" to genomics for medical scientists. To achieve this mission at the center, we have pronged our research activities into three parts as follows: (1) central support activities; (2) strategic and collaborative research activities; and (3) exploratory research activities aimed at new technology development. In particular, based on our previously developed single-cell analysis methods, we are integrating analyses in population context and along time axis to uncover new aspects of dynamics of the biological systems as the main exploratory research activities.

Research Fields

Molecular Biology & Genetics / Immunology

Research Subjects

• Single-cell analysis of protein secretion
• Technical development for proteomic and transcriptomic analyses of the immune system
• Construction of integrative genomics database

Publications

1. Shirasaki Y, Yamagishi M, Shimura N, Hijikata A, Ohara O. Shirasaki Y, Yamagishi M, Shimura N, Hijikata A, Ohara O.:
   "Toward an understanding of immune cell sociology: real-time monitoring of cytokine secretion at the single-cell level."
   IUBMB Life. 65, 28-34 (2012)
2. Kaji T, Ishige A, Hikida M, Taka J, Hijikata A, et al.:
   "Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory."
   J Exp Med. 209, 2079-2097 (2012)
3. Takezaki S, Yamada M, Kato M, Park MJ et al.:
   "Chronic Mucocutaneous Candidiasis Caused by a Gain-of-Function Mutation in the STAT1 DNA-Binding Domain."
   J Immunol. 189, 1521-1526 (2012)
4. Tofukuji S, Kuwahara M, Suzuki J, Ohara O, Nakayama T, Yamashita M.:
   "Identification of a New Pathway for Th1 Cell Development Induced by Cooperative Stimulation with IL-4 and TGF-β."
   J Immunol. 188, 4846-4857 (2012)
5. Takagi H, Fukaya T, Eizumi K, Sato Y, Sato K, et al.:
   "Plasmacytoid dendritic cells are crucial for the initiation of inflammation and T cell immunity in vivo."
   Immunity. 35, 958-971 (2011)
6. Murata Y, Yasumi T, Shirakawa R, Izawa K, Sakai H, et al.:
   "Rapid diagnosis of FHL3 by flow cytometric detection of intraplatelet Munc13-4 protein."
   Blood. 118, 1225-1230 (2011)
7. Motomura Y, Kitamura H, Hijikata A, Matsunaga Y, Matsumoto K, et al.:
   "The transcription factor E4BP4 regulates the production of IL-10 and IL-13 in CD4(+) T cells."
   Nat Immunol. 12, 450-459 (2011)
8. Ito M, Kitamura H, Kikuguchi C, Hase K, Ohno H, Ohara O.:
   "SP600125 inhibits cap-dependent translation independently of the c-Jun N-terminal kinase pathway."
   Cell Struct Funct. 36, 27-33 (2011)
9. Li X, Isono K, Yamada D, Endo TA, Endoh M, et al.:
   "Mammalian polycomb-like Pcl2/Mtf2 is a novel regulatory component of PRC2 that can differentially modulate polycomb activity both at the Hox gene cluster and at Cdkn2a genes."
   Mol Cell Biol. 31, 351-364 (2011)
10. Masuya H, Makita Y, Kobayashi N, Nishikata K, Yoshida Y, et al.:
    "The RIKEN integrated database of mammals."
    Nucleic Acids Res. 39, D861-870 (2011)