Structural Glycobiology Team

In order to elucidate the functions of the glycans attached to proteins, it is important to analyze the glycans from the structural point of view. Our team aim to reveal the functions of glycans through the three-dimensional structures of glycoproteins and oligosaccharide-related proteins. We prepare the glycoconjugates samples with the techniques of organic synthesis and cell-engineering, and determine the three-dimensional structures using biophysical methods such as NMR or X-ray crystallography. Furthermore, we construct databases of oligosaccharide structures using bioinformatics-based approaches and decode the 'glycosignals' from the structural information. We particularly focus on disease-related glycoproteins, and develop diagnostic and therapeutic agents.

Research Fields

Physics / Engineering / Chemistry / Biology & Biochemistry / Molecular Biology & Genetics / Immunology / Pharmacology & Toxicology / Clinical Medicine / Microbiology / Neuroscience & Behavior / Computer Science

Research Subjects

• Development of conformational analysis of glycans
• Recognition mode of glycan-binding molecules and their functions
• Construction of database on 3D structures of glycans

Publications

1. Hanashima, S., Fujiwara, N., Matsumoto, K., Iwasaki, N., Zheng, G., Torigoe, H., Suzuki, K., Taniguchi, N., and Yamaguchi, Y.:
   "Solution ¹⁷O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase"
   Chemical Communications, 49, 1449-1451 (2013)
2. Yamaguchi, Y., Nishima, W., Re, S., and Sugita, Y.:
   "Confident identification of isomeric N-glycan structures by combined ion mobility mass spectrometry and hydrophilic interaction liquid chromatography"
   Rapid Communications in Mass Spectrometry, 26 (24), 2877-2884 (2012)
3. Nagae, M., and Yamaguchi, Y.:
   "Function and 3D structure of the N-glycans on glycoproteins"
   International Journal of Molecular Sciences, 13(7), 8398-8429 (2012)
4. Subedi, G.P., Satoh, T., Hanashima, S., Ikeda, A., Nakada, H., Sato, R., Mizuno, M., Yuasa, N., Fujita-Yamaguchi, Y., and Yamaguchi, Y.:
   "Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector"
   Protein Expression and Purification, 82, 197–204(2012)
5. Hanashima, S., Kato, K., and Yamaguchi, Y.:
   "¹³C-NMR quantification of proton exchange at LewisX hydroxyl groups in water"
   Chemical Communications, 47, 10800-10802 (2011)
6. Kanagawa, M., Satoh, T., Ikeda, A., Adachi, Y., Ohno, N., and Yamaguchi, Y.:
   "Structural insights into recognition of triple-helical β-glucans by an insect fungal receptor"
   J. Biol. Chem., 286, 29158-29165 (2011)
7. Kanagawa, M., Satoh, T., Ikeda, A., Nakano, Y., Yagi, H., Kato, K., Kojima-Aikawa, K., and Yamaguchi, Y.:
   "Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related β-prism fold"
   Biochemical and Biophysical Research Communications, 404, 201-205 (2011)
8. Satoh, T., Chen, Y, Hu, D., Hanashima, S., Yamamoto, K., and Yamaguchi, Y.:
   "Structural basis for oligosaccharide recognition of misfolded glycoproteins by OS-9 in ER-associated degradation"
   Molecular Cell, 40, 905-916 (2010)
9. Hanashima, S., Sato, K., Naito, Y., Takematsu, H., Kozutsumi, Y., Ito, Y., and Yamaguchi, Y.:
   "Synthesis and binding analysis of unique AG2 pentasaccharide to human Siglec-2 using NMR techniques"
   Bioorganic & Medicinal Chemistry, 18, 3720-5 (2010)
10. Kato, K., Yamaguchi, Y., and Arata, Y.:
    "Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a model system"
    Progress in NMR Spectroscopy, 56, 346-359 (2010)