Plant Proteomics Research Unit

Plants have evolved unique adaptation systems to tolerate various environmental stresses. An understanding of the fundamental mechanisms underlying adaptation processes is expected to provide novel ideas for improving plant functions. The main goal of our unit is to dissect plant specific signaling networks essential for the adaptation system by employing advanced proteomics methods.

Research Fields

Biology & Biochemistry / Molecular Biology & Genetics / Agricultural Sciences / Plant & Animal Science

Research Subjects

• Method development for post-translational modification analysis
• Dissection of plant immune signaling by proteomics

Publications

1. Nakagami, H., Sugiyama, N., Ishihama, Y., and Shirasu K.:
   "Shotguns in the front line: phosphoproteomics in plants"
   Plant Cell Physiol. Jan;53(1):118-24. (2012).
2. Joshi, HJ., Hirsch-Hoffmann, M., Baerenfaller, K., Gruissem, W., Baginsky, S., Schmidt, R., Schulze, WX., Sun, Q., van Wijk, KJ., Egelhofer, V., Wienkoop, S., Weckwerth, W., Bruley, C., Rolland, N., Toyoda, T., Nakagami, H., Jones, AM., Briggs, SP., Castleden, I., Tanz, SK., Millar, AH., and Heazlewood, JL.:
   "MASCP Gator: An Aggregation Portal for the Visualization of Arabidopsis Proteomics Data"
   Plant Physiol. Jan;155(1):259-70. (2011).
3. Nakagami, H., Sugiyama, N., Mochida, K., Daudi, A., Yoshida, Y., Toyoda, T., Tomita, M., Ishihama, Y., and Shirasu, K.:
   "Large-scale comparative phosphoproteomics identifies conserved phosphorylation sites in plants"
   Plant Physiol. Jul;153(3):1161-74. (2010)
4. Sugiyama, N., Nakagami, H., Mochida, K., Daudi, A., Tomita, M., Shirasu, K., and Ishihama, Y.:
   "Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis"
   Mol Syst Biol. 2008;4:193. (2008)
5. Djamei, A., Pitzschke, A., Nakagami, H., Rajh, I., and Hirt, H.:
   "Trojan horse strategy in Agrobacterium transformation: abusing MAPK defense signaling"
   Science. Oct19;318(5849):453-6. (2007)
6. Nakagami, H., Soukupová, H., Schikora, A., Zárský, V., and Hirt, H.:
   "A Mitogen-activated protein kinase kinase kinase mediates reactive oxygen species homeostasis in Arabidopsis"
   J Biol Chem. Dec15;281(50):38697-704. (2006)
7. Veronese, P., Nakagami, H., Bluhm, B., Abuqamar, S., Chen, X., Salmeron, J., Dietrich, RA., Hirt, H., and Mengiste, T.:
   "The membrane-anchored BOTRYTIS-INDUCED KINASE1 plays distinct roles in Arabidopsis resistance to necrotrophic and biotrophic pathogens"
   Plant Cell. Jan;18(1):257-73. (2006)
8. Nakagami, H., Kiegerl, S., and Hirt, H.:
   "OMTK1, a novel MAPKKK, channels oxidative stress signaling through direct MAPK interaction"
   J Biol Chem. Jun25;279(26):26959-66. (2004)
9. Nakagami, H., Kawamura, K., Sugisaka, K., Sekine, M., and Shinmyo, A.:
   "Phosphorylation of retinoblastoma-related protein by the cyclin D/cyclin-dependent kinase complex is activated at the G1/S-phase transition in tobacco"
   Plant Cell. Aug;14(8):1847-57. (2002)
10. Nakagami, H., Sekine, M., Murakami, H., and Shinmyo, A.:
    "Tobacco retinoblastoma-related protein phosphorylated by a distinct cyclin-dependent kinase complex with Cdc2/cyclin D in vitro"
    Plant J. May;18(3):243-52. (1999)