Molecular Ligand Target Research Team

Small molecular ligands with unique activities must have the specific target molecules that exist in their cells or organisms. Identification of the target molecules is critical for elucidating the mode of action of the molecular ligands and for drug development. However, drug target identification has been difficult in general, because the mode of interactions between the molecular ligands and their targets is not uniform. Our team aims at developing innovative techniques based on global analysis of yeast genetic interaction, which leads to quick and accurate detection of the ligand-target interactions.

Research Fields

Chemistry / Biology & Biochemistry / Molecular Biology & Genetics / Pharmacology & Toxicology / Plant & Animal Science / Microbiology

Research Subjects

• Global analysis of genetic interaction between molecular ligands and their target molecules
• Mode of action of bioactive compounds

Publications

1. Andrusiak, K., Piotrowski, JS., and Boone, C.
   "Chemical-genomic profiling: systematic analysis of the cellular targets of bioactive molecules."
   Bioorg, Med. Chem. 20: 1952-1960, 2012
2. Williams, DE., Dalisay, DS., Patrick, BO., Matainaho T., Andrusiak, K., Deshpande, R., Myers, CL., Piotrowski, JS., Boone, C., Yoshida, M., and Andersen, RJ.
   "Padanamides A and B, highly modified linear tetrapeptides produced in culture by a Streptomyces sp. isolated from marine sediment collected in Papua New Guinea."
   Org. Lett., 13: 3936-3939, 2011.
3. Arita, Y., Nishimura, S., Matsuyama, A., Yashiroda, Y., Usui, T., Boone, C., and Yoshida, M.
   "Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome."
   Mol. Biosyst., 7: 1463-1472, 2011.
4. Piotrowski, JS., Ho, CH., and Boone, C.
   "The awesome power of synergy from chemical-chemical profiling."
   Chem. Biol., 17: 789-790, 2010.
5. Nishimura, S., Arita, Y., Honda, M., Iwamoto, K., Matsuyama, A., Shirai, A., Kawasaki, H., Kakeya, H., Kobayashi, T., Matsunaga, S., and Yoshida, M.
   "Marine antifungal theonellamides target 3beta-hydroxysterol to activate Rho1 signaling."
   Nat. Chem. Biol., 6: 519-526, 2010.
6. Ho, CH., Magtanong, L., Barker, SL., Gresham, D., Nishimura, S., Natarajan, P., Koh, JL., Porter, J., Gray, CA., Andersen, RJ., Giaever, G., Nislow, C., Andrews, B., Botstein, D., Graham, TR., Yoshida, M., and Boone, C.
   "A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds."
   Nat. Biotechnol., 27: 369-377, 2009.