NMR Facility Support Unit

In "open advanced research facilities/NMR platform initiative" program by MEXT, we provide access in the RIKEN large-scale NMR facility and technical supports on NMR applications for researchers including industrial applicants, to promote innovative researches in science and technology. In "open NMR platform initiative" programs, we will structure advanced supporting research infrastructures as a representative organization of the NMR network in Japan.

Research Fields

Chemistry / Materials Sciences / Biology & Biochemistry / Molecular Biology & Genetics / Pharmacology & Toxicology / Agricultural Sciences

Publications

1. * Stanley, D. J., Bartholomeeusen. K., Crosby, D. C., Kim, D. Y., Kwon, E., Yen, L., Cartozo, N. C., Li, M., Jäger, S., Mason-Herr, J., Hayashi. F., Yokoyama, S., Krogan, N. J., Harris, R. S., Peterlin, B. M., and Gross, J. D.:
   "Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G."
   PLoS Pathog. 8,　e1003085. doi: 10.1371/journal.ppat.1003085. Epub 2012 Dec 27.
2. Matsumori, N., Hiradate, Y., Shibata, H., Oishi, T., Simma, S., Toyoda, M., Hayashi, F., Yoshida, M, Murata, M, and Morisawa, M.:
   "A Novel Sperm-Activating and Attracting Factor (SAAF) from the Ascidian Ascidia sydneiensis"
   Organic Letters, 15, 294-297 (2013).
3. Matsumura, K., Hayashi, F., Nagashima, T. and Hyon, S. H.:
   "Long-term cryopreservation of human mesenchymal stem cells using carboxylated poly-l-lysine without the addition of proteins or dimethyl sulfoxide."
   J. Biomater. Sci. Polymer Edn. DOI:10.1080/09205063.2013.771318
4. Roa, A., Hayashi, F., Yang, Y., Lienlaf, M., Zhou, J., Shi, J., Watanabe, S., Kigawa, T., Yokoyama, S., Aiken, C., and Diaz-Griffero, F.:
   "RING domain mutations uncouple TRIM5α restriction of HIV-1 from inhibition of reverse transcription and acceleration of uncoating."
   J. Virol. 86, 1717-27 (2012).
5. Lienlaf, M., Hayashi, F., Di Nunzio, F., Tochio, N., Kigawa, T., Yokoyama, S., and Diaz-Griffero, F.:
   "Contribution of E3-ubiquitin ligase activity to HIV-1 restriction by TRIM5alpha(rh): structure of the RING domain of TRIM5alpha."
   J. Virol. 85, 8725-37 (2011).
6. Nagano, M., Hoshino, D., Koshiba, S., Shuo, T., Koshikawa, N., Tomizawa, T., Hayashi, F., Tochio, N., Harada, T., Akizawa, T., Watanabe, S., Handa, N., Shirouzu, M., Kigawa, T., Yokoyama, S., and Seiki, M.:
   "ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain."
   J Biol Chem. 286, 31598-609 (2011).
7. Arakawa, A., Handa, N., Ohsawa, N., Shida, M., Kigawa, T., Hayashi, F., Shirouzu, M., and Yokoyama, S.:
   "The C-terminal BAG domain of BAG5 induces conformational changes of the Hsp70 nucleotide-binding domain for ADP-ATP exchange."
   Structure. 18, 309-19 (2010).
8. Diaz-Griffero, F., Qin, XR., Hayashi, F., Kigawa, T., Finzi, A., Sarnak, Z., Lienlaf, M., Yokoyama, and S., Sodroski, J.:
   "A B-box 2 surface patch important for TRIM5alpha self-association, capsid binding avidity, and retrovirus restriction."
   J. Virol. 83, 10737-51 (2009).
9. * Furukawa, A., Nagata, T., Matsugami, A., Habu, Y., Sugiyama, R., Hayashi, F., Kobayashi, N., Yokoyama, S., Takaku, H., and Katahira, M.:
   "Structure, interaction and real-time monitoring of the enzymatic reaction of wild-type APOBEC3G."
   EMBO J. 28, 440-51 (2009).
10. Li, H., Koshiba, S., Hayashi, F., Tochio, N., Tomizawa, T., Kasai, T., Yabuki, T., Motoda, Y., Harada, T., Watanabe, S., Inoue, M., Hayashizaki, Y., Tanaka, A., Kigawa, T., and Yokoyama, S.:
    "Structure of the C-terminal phosphotyrosine interaction domain of Fe65L1 complexed with the cytoplasmic tail of amyloid precursor protein reveals a novel peptide binding mode."
    J Biol Chem. 283, 27165-78 (2008).