Cellular Function Conversion Technology Team

We will develop technologies for direct cell conversion to target cell types of potential therapeutic interest, based on the integrated knowledge of the transcriptome status, the transcriptional basins, the epigenome, and the non-coding RNA, including technologies for high efficient, loci specific epigenome modification. Linked to the direct cell conversion, differentiation and safety of stem cells will be explored.

Research Fields

Biology & Biochemistry / Molecular Biology & Genetics

Research Subjects

• Development of cell function manipulation technologies
• Transcription Regulation Network Analysis in Mammals
• Promoter analysis of drug effect

Publications

1. H. Suzuki, A. R. Forrest, E. van Nimwegen et al. "The transcriptional network that controls growth arrest , differentiation in a human myeloid leukemia cell line", Nat Genet, 41, 553-562 (2009)
2. Ravasi T, Suzuki H, Cannistraci CV, Katayama S, Bajic VB et al. "An atlas of combinatorial transcriptional regulation in mouse and man", Cell. 2010 Mar 5;140(5):744-52.
3. Suzuki T, Nakano-Ikegaya M, Yabukami-Okuda H, de Hoon M, Severin J, Saga-Hatano S, Shin J, Kubosaki A, Simon C, Hasegawa Y, Hayashizaki Y, Suzuki H. "Reconstruction of Monocyte Transcriptional Regulatory Network Accompanies Monocytic Functions in Human Fibroblasts.", PLoS One. 2012, 7(3), e33474.
4. Shin JW, Suzuki T, Ninomiya N, Kishima M, Hasegawa Y, Kubosaki A, Yabukami H, Hayashizaki Y, Suzuki H. "Establishment of single-cell screening system for the rapid identification of transcriptional modulators involved in direct cell reprogramming.", Nucleic Acids Res. 40(21):e165 (2012).
5. Hasegawa Y, Takahashi N, Forrest AR, Shin JW, Kinoshita Y, Suzuki H, Hayashizaki Y. "CC chemokine ligand 2 and leukemia inhibitory factor cooperatively promote pluripotency in mouse induced pluripotent cells." Stem Cells. 2011 Aug;29 (8): 1196-205.
6. Schroder K, Irvine KM, Taylor MS, Bokil NJ, Le Cao KA, Masterman KA, Labzin LI, Semple CA, Kapetanovic R, Fairbairn L, Akalin A, Faulkner GJ, Baillie JK, Gongora M, Daub CO, Kawaji H, McLachlan GJ, Goldman N, Grimmond SM, Carninci P, Suzuki H, Hayashizaki Y, Lenhard B, Hume DA, Sweet MJ. "Conservation and divergence in Toll-like receptor 4-regulated gene expression in primary human versus mouse macrophages." Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):E944-53.
7. Vitezic M, Lassmann T, Forrest AR, Suzuki M, Tomaru Y, Kawai J, Carninci P, Suzuki H, Hayashizaki Y, Daub CO. "Building promoter aware transcriptional regulatory networks using siRNA perturbation" , deepCAGE. Nucleic Acids Res. 2010 Dec 1;38(22):8141-8.
8. Forrest AR, Kanamori-Katayama M, Tomaru Y, Lassmann T, Ninomiya N, Takahashi Y, de Hoon MJ, Kubosaki A, Kaiho A, Suzuki M, Yasuda J, Kawai J, Hayashizaki Y, Hume DA, Suzuki H. "Induction of microRNAs, mir-155, mir-222, mir-424 , mir-503, promotes monocytic differentiation through combinatorial regulation." Leukemia. 2010 Feb;24(2):460-6. Epub 2009 Dec 3.
9. C. Ogawa, K. Usui, F. Ito, M. Itoh, Y. Hayashizaki, , H. Suzuki, "Role of survival motor neuron complex components in small nuclear ribonucleoprotein assembly", J Biol Chem, 284,14609-17 (2009)
10. K. Usui, T. Maki, F. Ito, A. Suenaga, S. Kidoaki, M. Itoh, M. Taiji, T. Matsuda, Y. Hayashizaki, and H. Suzuki, "Nanoscale elongating control of the self-assembled protein filament with the cysteine-introduced building blocks", Protein Sci, 18, 960-969 (2009)