Sequencer Technology Team

Human beings are composed of various types of heterogeneous cells, which have been differentiated from single egg cell originally. To investigate the heterogeneity of the differentiation process, diseased cells status and drug responses etc, the establishment of true single cell biology based on the single cell database is more significant than the use of assembly of mixture from heterogeneous cells. We have been developing non-biased direct true single cell sequencing system, which has no amplification process (PCR) with capturing the target sample (DNA, RNA, modification on DNA or RNA) in a single cell with very high efficiency to achieve these goals.

Research Fields

Physics / Engineering / Chemistry / Materials Sciences / Biology & Biochemistry / Molecular Biology & Genetics / Pharmacology & Toxicology / Clinical Medicine

Research Subjects

• Development of single cell sequencer
• Development of single cell preparation methods
• Development of single cell data analysis

Publications

1. *Tsai, A., Petrov, A., Marshall, R. A., Korlach, J., Uemura, S*., and Puglisi, J. D*.: *corresponding author
   "Heterogeneous pathways and timing of factor departure during translation initiation"
   Nature 487, 390-393 (2012)
2. *Masuda, T., Petrov, A., Iizuka, R., Funatsu, T., Puglisi, J. D. and Uemura, S.:
   "Initiation factor 2, tRNA and 50S subunits cooperatively stabilize mRNAs on the ribosome during initiation"
   Proc. Natl. Acad. Sci. U.S.A. 109, 4881-4885 (2012)
3. *Petrov, A., Kornberg, G., O'Leary, S., Tsai, A., Uemura, S. and Puglisi, J. D.:
   "Dynamics of the translational machinery"
   Curr. Opin. Struct. Biol. 21, 137-145 (2011)
4. *Uemura, S., Aitken, C. E., Korlach, J., Flusberg, B., Turner, S. and Puglisi, J. D.:
   "Real time tRNA transit on single translating ribosomes at codon resolution"
   Nature 464, 1012-1017 (2010)
5. *Uemura, S., Iizuka, R., Ueno, T., Shimizu, Y., Taguchi, H., Ueda, T., Puglisi, J. D. and Funatsu, T.:
   "Single molecule imaging of full protein synthesis by immobilized ribosomes"
   Nucleic Acids Research 36, e70 (2008)
6. *Uemura, S., Dorywalska, M., Lee, T. H., Kim, H. D., Puglisi, J. D. and Chu, S.:
   "Peptide bond formation destabilizes Shine-Dalgarno interaction on the ribosome"
   Nature 446, 454-457 (2007)
7. *Uemura, S., Higuchi, H., Olivares, A. O., De La Cruz, E. M. and Ishiwata, S.:
   "Mechanochemical coupling of two substeps in the single molecule of myosin-V"
   Nature Struct. & Mol. Biol. 11, 877-883 (2004)
8. *Uemura, S. and Ishiwata, S.:
   "Loading direction regulates the affinity of ADP for kinesin"
   Nature Struct. Biol. 10, 308-311 (2003)
9. *Ali, M. Y., Uemura, S., Adachi, K., Itoh, H., Kinosita. K. Jr, and Ishiwata, S.:
   "Myosin-V is a left-handed spiral motor on the right-handed actin helix"
   Nature Struct. Biol. 9, 464-467 (2002)
10. *Uemura, S., Kawaguchi, K., Yajima, J., Edamatsu, M., Toyoshima, Y. Y. and Ishiwata, S.:
    "Kinesin-microtubule binding depends on both nucleotide state and loading direction"
    Proc. Natl. Acad. Sci. U.S.A. 99, 5977-5981 (2002)