Bioengineering Laboratory

The principal purpose of the laboratory is to explore a new frontier of research field which fuses engineering and biological science. On the basis of polymer chemistry, analytical chemistry, surface chemistry, biochemistry and molecular biology, we are studying on new materials comprising biological components, novel methodology for bioanalysis and medical diagnosis, and artificial systems for regulation of biological processes. Those new ideas and materials are being applied to the field of biomaterials science, environmental science, micro/nanoscience, life science and medical engineering. For example, we newly prepared DNA-vinyl polymer conjugates which have been applied for DNA biosensor, affinity electrophoresis, SNPs-responsive diagnostic nanoparticle, gene-responsive hydrogel, artificial gene regulation system, etc. These researches may be classified into a new category, i.e., "DNA engineering".

Research Subjects

• DNA Conjugate Chemistry
• Bioanalytical Systems Engineering
• Biomacromolecular Science

Publications

1. Sakono, M., Utsumi, A., Zako, T., Abe, T., Yohda, M., and Maeda, M.:
   "Formation of non-toxic Abeta fibrils by small heat shock protein under heat-stress condition"
   Biochem. Biophys. Res. Commun. 430, 1259-1264 (2013)
2. Arata H., Komatsu H., Hosokawa, K, Maeda, M.:
   "Rapid and sensitive microRNA detection with laminar flow-assisted dendritic amplification on power-free microfluidic chip"
   PLoS ONE, 7, e48329, (2012)
3. Han, A., Hosokawa, K., and Maeda, M.:
   "Activity Measurement of Protein Kinase and Protein Phosphatase by Microchip Phosphate-Affinity Electrophoresis"
   Anal. Biochem., 421, 782-784 (2012)
4. Hiraishi, T., Yamashita, K., Sakono, M., Nakanishi, J., Tan, L. Z., Sudesh, K., Abe, H., and Maeda, M.:
   "Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface"
   Macromol. Biosci., 12, 218-224 (2012)
5. Kundu, L. M., Tsukada, H., Matsuoka, Y., Kanayama, N., Takarada, T., and Maeda, M.:
   "Estimation of Binding Constants of Peptide Nucleic Acid and Secondary-Structured DNA by Affinity Capillary Electrophoresis"
   Anal. Chem., 84, 5204-5209 (2012)
6. Ooi, W. Y., Fujita, M., Pan, P., Tang, H. Y., Sudesh, K., Ito, K., Kanayama, N., Takarada, T., and Maeda, M.:
   "Structural Characterization of Nanoparticles from Thermoresponsive Poly(N-isopropylacrylamide)-DNA Conjugate"
   J Colloid Int. Sci., 374, 315-320 (2012)
7. Pan, P., Fujita, M., Ooi. W.Y., Sudesh, K., Takarada, T., Goto, A., and Maeda, M.:
   "Thermoresponsive Micellization and Micellar Stability of Poly(N-isopropylacrylamide)-b-DNA Diblock and Miktoarm Star Polymers"
   Langmuir, 28,14347-14356 (2012)
8. Zako, T., Sakono, M., Kobayashi, T., Sorgjerd, K., Nilsson, K. P., Hammarstrom, P., Lindgren, M., and Maeda, M.:
   "Cell Interaction Study of Amyloid Using Luminescent Conjugated Polythiophene: Implication that Amyloid Cytotoxicity is Correlated with Prolonged Cellular Binding"
   ChemBioChem, 13, 358-363 (2012)
9. Kanayama, N., Takarada, T., and Maeda, M.:
   "Rapid naked-eye detection of mercury ion based on non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles"
   Chem. Commun. 47, 2077-2079 (2011)
10. Ozasa, K., Lee, J., Song, S., Hara, M., and Maeda, M.:
    "Two-Dimensional Optical Feedback Control of Euglena Confined in Closed-Type Microfluidic Channels"
    Lab Chip, 11, 1933-1940 (2011)