Mass Spectrometry Analysis Unit

The identification of the individual components of molecular complexes and elucidation of their structures can provide fundamental insights into the intricate mechanisms of development and regeneration. The Mass Spectrometry Analysis subunit uses LC-MASS spectrometry, a technology that enables the high-speed analysis of protein components from even minute biological samples. The subunit undertakes the analysis of protein modifications as well.

Publications

1. Terabayashi, T., Sakaguchi, M., Shinmyozu, K., Ohshima, T., Johjima, A., Ogura, T., Miki, H., Nishinakamura, R.:
   "Phosphorylation of Kif26b promotes its polyubiquitination and subsequent proteasomal degradation during kidney development."
   PLoS One. 7, e39714. (2012)
2. Shiomi, Y., Hayashi, A., Ishii, T., Shinmyozu, K., Nakayama, J., Sugasawa, K., Nishitani, H.:
   "Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation."
   Mol Cell Biol. 32, 2279-2288. (2012)
3. McCloskey, A., Taniguchi, I., Shinmyozu, K., Ohno, M.:
   "hnRNP C tetramer measures RNA length to classify RNA polymerase II transcripts for export."
   Science. 335, 1643-1646. (2012)
4. Satoh, R., Tanaka, A., Kita, A., Morita, T., Matsumura, Y., Umeda, N., Takada, M., Hayashi, S., Tani, T., Shinmyozu, K., Sugiura, R.:
   "Role of the RNA-binding protein Nrd1 in stress granule formation and its implication in the stress response in fission yeast."
   PLoS One. 7, e29683. (2012)
5. Hatanaka, A., Chen, B., Sun, J,Q., Mano, Y., Funakoshi, M., Kobayashi, H., Ju, Y., Mizutani, T., Shinmyozu, K., Nakayama, J., Miyamoto, K., Uchida, H., Oki, M.:
   "Fub1p, a novel protein isolated by boundary screening, binds the proteasome complex."
   Genes Genet Syst. 86, 305-314. (2011)
6. Zou, P., Yoshihara, H., Hosokawa, K., Tai, I., Shinmyozu, K., Tsukahara, F., Maru, Y., Nakayama, K., Nakayama, KI., Suda, T.:
   "p57(Kip2) and p27(Kip1) cooperate to maintain hematopoietic stem cell quiescence through interactions with Hsc70."
   Cell Stem Cell. 9, 247-261. (2011)
7. Kitano, E., Hayashi, A., Kanai, D., Shinmyozu, K., Nakayama, J.:
   "Roles of fission yeast Grc3 protein in ribosomal RNA processing and heterochromatic gene silencing."
   J Biol Chem. 286, 15391-15402. (2011)
8. Otani, T., Oshima, K., Onishi, S., Takeda, M., Shinmyozu, K., Yonemura, S. and Hayashi, S.:
   "IKKɛ Regulates Cell Elongation through Recycling Endosome Shuttling."
   Dev Cell. 20, 219-232. (2011)
9. Hiragami-Hamada, K., Shinmyozu, K., Hamada, D., Tatsu, Y., Uegaki, K., Fujiwara, S. and Nakayama, J.:
   "N-Terminal Phosphorylation of HP1{alpha} Promotes Its Chromatin Binding."
   Mol Cell Biol. 31, 1186-1200. (2011)
10. Shirai, A., Sadaie, M., Shinmyozu, K. and Nakayama, J.:
    "Methylation of ribosomal protein L42 regulates ribosomal function and stress-adapted cell growth."
    J Biol Chem. 285, 22448-22460. (2010)