Optical Image Analysis Unit

Bio-imaging is an interdisciplinary process that integrates molecular cell biology and biochemistry, as well as technology from optics, engineering, and computer sciences. It has enabled scientists to visualize biological processes at the cellular and molecular levels and today, has become an indispensable field for research on biological systems. We aim to design an imaging environment that can adapt to changing scientific demands and can contribute to the progress of biology. We hope to accelerate biological research by matching research concepts with the latest technologies from the different specialized fields. In addition, we aims to shed light on the molecular mechanisms controlling the microtubule cytoskeleton in the cell/tissue morphogenesis by making full use of the available technology.

Research Subjects

• Development of method allowing high resolution in vivo imaging

Publications

1. Shimozawa T, Yamagata K, Kondo T, Hayashi S, Shitamukai A, Konno D, Matsuzaki F, Takayama J, Onami S, Nakayama H, Kosugi Y, Watanabe TM, Fujita K, Mimori-Kiyosue Y.:
   "Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging."
   Proc Natl Acad Sci U S A. 2013 Feb 11. [Epub ahead of print]
2. Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y.:
   "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells."
   PLoS One. 2012;7(12):e51442. doi: 10.1371/journal.pone.0051442. Epub 2012 Dec 12.
3. Mimori-Kiyosue Y.:
   "Shaping microtubules into diverse patterns: molecular connections for setting up both ends."
   Cytoskeleton (Hoboken). 2011 Nov;68(11):603-18.
4. Hotta A, Kawakatsu T, Nakatani T, Sato T, Matsui C, Sukezane T, Akagi T, Hamaji T, Grigoriev I, Akhmanova A, Takai Y, Mimori-Kiyosue Y.:
   "Laminin-based cell adhesion anchors microtubule plus ends to the epithelial cell basal cortex through LL5alpha/beta."
   J Cell Biol. 2010 May 31;189(5):901-17.
5. Matsui C, Kaieda S, Ikegami T, Mimori-Kiyosue Y.:
   "Identification of a link between the SAMP repeats of adenomatous polyposis coli tumor suppressor and the Src homology 3 domain of DDEF."
   J Biol Chem. 283(47):33006-33020. 2008.
6. Mimori-Kiyosue Y, Grigoriev I, Lansbergen G, Sasaki H, Matsui C, Severin F, Galjart N, Grosveld F, Vorobjev I, Tsukita S, Akhmanova A.:
   "CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex."
   J Cell Biol. 168(1):141-153. 2005.
7. Mimori-Kiyosue Y, Tsukita S.:
   ""Search-and-capture" of microtubules through plus-end-binding proteins (+TIPs)."
   J Biochem (Tokyo).134(3):321-326. 2003.
8. Mimori-Kiyosue Y, Tsukita S.:
   "Where is APC going?"
   J Cell Biol. 154(6):1105-1109. 2001.
9. Mimori-Kiyosue Y, Shiina N, Tsukita S.:
   "The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules."
   Curr Biol. 10(14):865-868. 2000.
10. Mimori-Kiyosue Y, Shiina N, Tsukita S.:
    "Adenomatous polyposis coli (APC) protein moves along microtubules and concentrates at their growing ends in epithelial cells."
    J Cell Biol. 148(3):505-518. 2000.