Genetic Engineering Unit

Quality and efficacy of the research are greatly dependent on how easily mutant mice are handled. The major aim of this laboratory is to develop mutant mice useful for researches in Developmental Biology and Regenerative Medicinebyin collaboration with researchers not only in CDB but also in Japan and Asian. With the sequence information on genes from researchers, the construction of vectors, ES culture, isolation of homologous recombinants, production of chimera as well as generation of transgenic miceare all being done speedily by this laboratory. Special focus is in development of mutant mice, embry oculture and microscopic techniques for bioimaging in mouse embryos.

Research Subjects

• Generation of mouse mutants useful for developmental biology and regenerative medicine
• Development of mutant mice and techniques for bioimaging in mouse embryos
• Development of technology to manipulate mouse embryos genetically and embryologically

Publications

1. Abe T, Sakaue-Sawano A, Kiyonari H, Shioi G, Inoue K, Horiuchi T, Nakao K, Miyawaki A, Aizawa S, Fujimori T.:
   "Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter."
   Development. 140(1):237-246, 2013
2. Shinohara R, Thumkeo D, Kamijo H, Kaneko N, Sawamoto K, Watanabe K, Takebayashi H, Kiyonari H, Ishizaki T, Furuyashiki T, Narumiya S.:
   "A role for mDia, a Rho-regulated actin nucleator, in tangential migration of interneuron precursors."
   Nature Neuroscience. 15: 373-380, 2012
3. Fukuhara S, Simmons S, Kawamura S, Inoue A, Orba Y, Tokudome T, Sunden Y, Arai Y, Moriwaki K, Ishida J, Uemura A, Kiyonari H, Abe T, Fukamizu A, Hirashima M, Sawa H, Aoki J, Ishii M, Mochizuki N.:
   "The sphingosine-1-phosphate transporter Spns2 expressed on endothelial cells regulates lymphocyte trafficking in mice."
   The Journal of Clinical Investigation.122(4), 1416-1426, 2012
4. Okuda-Ashitaka E, Minami T, Tsubouchi S, Kiyonari H, Iwamatsu A, Noda T, Hanada H and Ito S.:
   "Identification of NIPSNAP1 as a nocistatin-interacting protein involving pain transmission."
   J. Biol. Chem. 287(13), 10403-10413, 2012
5. Aoyama K, Wang F, Matsumura N, Kiyonari H, Shioi G, Tanaka K, Kinoshita C, Kikuchi-Utsumi K, Watabe M, Nakaki T.:
   "Increased neuronal glutathione and neuroprotection in GTRAP3-18-deficient mice."
   Neurobiol Dis. 45(3), 973-982, 2012
6. Niisato E, Nagai J, Yamashita N, Abe T, Kiyonari H, Goshima Y and Ohshia T.:
   "CRMP4 suppresses apical dendrite bifurcation of CA1 pyramidal neurons in the mouse hippocampus."
   Developmental Neurobiology. 72(11), 1447-1457, 2012
7. Tao H, Inoue K, Kiyonari H, Bassuk AG, Axelrod JD, Sasaki H, Aizawa S, Ueno N.:
   "Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis."
   Dev Biol. 364(2), 138-148, 2012
8. Kanemaru K, Nakamura Y, Sato K, Kojima R, Takahashi S, Yamaguchi M, Ichinohe M, Kiyonari H, Shioi G, Kabashima K, Nakahigashi K, Asagiri M, Jamora C, Yamaguchi H, Fukami K.:
   "Epidermal phospholipase Cδ1 regulates granulocyte counts and systemic interleukin-17 levels in mice."
   Nat Commun. 2012 jul 17;3:393
9. Nishimur R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G, Yamaguchi A, Tsumaki N, Akiyama H, Yoneda T.:
   "Osterix Regulates Calcification and Degradation of Chondrogenic Matrices Through Matrix Metalloproteinase(MMP13) Expression in Association with Transcription Factor Runx2 During Endochondral Ossification."
   J Biol Chem. 287(40), 33179-33190, 2012
10. Inazuka F, Sugiyama N, Tomita M, Abe T, Shioi G, Esumi H.:
    "Muscle-specific knock-out of NUAK family SNF1-like kinase 1(NUAK1)prevents high fat diet-induced glucose intolerance."
    J Biol Chem. 287(20), 16379-16389, 2012