Animal Resource Unit

Our laboratory provides support services for the care of mice and rats in SPF conditions and the receipt of mutant mice from institutes in Japan and overseas, as well as reproductive services including cleaning and colony establishment through IVF, and lineage preservation by vitrification cryopreservation. We also provide pregnant mice, embryos, and surrogates to support animal research in the CDB, and develop tools and techniques for use in rodent reproductive biotechnology.

Research Subjects

• Cryopreservation
• Development of mouse reproduction technology

Publications

1. Nakao, K. and Inoue, K.:
   "Cryopreservation for Broader Production of Transgenic Mice by DNA Injection into Zygotes."
   Exp. Anim. 59(2), 225-229(2010).
2. Kamiya D, Banno S, Sasai N, Ohgushi M, Inomata H, Watanabe K, Kawada M, Yakura R, Kiyonari H, Nakao K, Jakt LM, Nishikawa S, Sasai Y.:
   "Intrinsic transition of embryonic stem-cell differentiation into neural progenitors"
   Nature.24:503-509(2011)
3. Abe T, Kiyonari H, Shioi G, Inoue K, Nakao K, Aizawa S, Fujimori T.:
   "Establishment of conditional reporter mouse lines at ROSA26locus for live cell imaging."
   Genesis. 49:579-590, (2011)
4. Shioi G, Kiyonari H, Abe T, Nakao K, Fujimori T, Jang CW, Huang CC, Akiyama H, Behringer RR, Aizawa S.:
   "A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging."
   Genesis. 49:570-578, (2011)
5. Doi M, Ishida A, Miyake A, Sato M, Komatsu R, Yamazaki F, Kimura I, Tsuchiya S, Kori H, Seo K, Yamaguchi Y, Matsuo M, Fustin JM, Tanaka R, Santo Y, Yamada H, Takahashi Y, Araki M, Nakao K, Aizawa S, Kobayashi M, Obrietan K, Tsujimoto G, Okamura H.:
   "Circadian regulation of intracellular G-protein signalling mediates intercellular synchrony and rhythmicity in the suprachiasmatic nucleus."
   Nature Communications, 327: 1316-1324, (2011)