Technology and Development Team for Mouse Phenotype Analysis

The Technology and Development Team for Mouse Phenotype Analysis develop a systematic and comprehensive phenotyping platform consisting of more than 400 test items, based on the knowledge of pathogenesis of human diseases. Using this system, we perform more comprehensive and profound phenotyping of mouse mutants, which are and will be deposited to RIKEN BRC. Judging from the results of preceding foreign phenotyping projects, we expect that existing mouse mutants be revalued as new disease models by identified novel phenotypes. We also develop a standard operating procedures (SOP) of mouse phenotyping and exchange this information with attending the IMPC (International Mouse Phenotyping Consortium) projects for analyzing international public knockout mouse resources, and thereby we will contribute to international efforts for standardization of mouse phenotype data. Finally, through collaborations with and our team will integrate information of mouse phenotypes and clinical features of human diseases. It will enable more objective characterization of mouse mutants, and lead to the development of high value-added mouse resources.

Research Subjects

• Development of a systematic and comprehensive mouse phenotyping platform.
• Development of standard operating procedures (SOP) of mouse phenotyping
• International contribution for standerized mouse phenotyping in IMPC
• The development of high value-added mouse resources corresponding with human disease information

Publications

1. Gotoh H., Hirawatari K., Hanzawa N., Miura I., Wakana S.:
   "QTL on mouse chromosomes 1 and 4 causing sperm-head morphological abnormality and male subfertility"
   Mammalian Genome, 23(7-8): 399-403 (2012)
2. Furuse T., Yamada I., Kushida T., Masuya H., Miura I., Kaneda H., Kobayashi K., Wada Y., Yuasa S., Wakana S.:
   "Behavioral and euromorphological characterization of a novel Tuba1 mutant mouse"
   Behavioural Brain Research, 216(1) 167-174 (2012)
3. Ohnishi T., Yamada K., Watanabe A., Ohba H., Sakaguchi T., Honma Y., Iwayama Y., Toyota T., Maekawa M., Watanabe K., Detera-Wadleigh S.D., Wakana S., Yoshikawa T.
   "Ablation of Mrds1/Ofcc1 Induces Hyper-c-Glutamyl Transpeptidasemia without Abnormal Head Development and Schizophrenia-Relevant Behaviors in Mice"
   PLos ONE, 6(12) e29499 (2011)
4. KohdaT., Ogonuki N., Inoue K., Furuse T., Kaneda H., Suzuki T., Kaneko-Ishino T., Wakayama T., Wakana S., Ogura A.:
   "Fumitoshi Ishino: Intracytoplasmic sperm injection induces transcriptome perturbation without any transgenerational effect"
   Biochemical and Biophysical Research Communications, 410(2): 282-288 (2011)
5. Hayasaka N., Aoki K., Kinoshita S., Yamaguchi S., Wakefield J.K., Tsuji-Kawahara S., Horikawa K., Ikegami H., Wakana S., Murakami T., Ramabhadran R., Miyazawa M., Shibata S.:
   "Attenuated food anticipatory activity and abnormal circadian locomotor rhythms in Rgs16 knockdown mice"
   PLos ONE, 6(3): e17655-1-7 (2011)
6. Wada Y., Furuse T., Yamada I., Masuya H., Kushida T., Shibukawa Y., Nakai Y., Kobayashi K., Kaneda H., Gondo Y., Noda T., Shiroishi T., Wakana S.:
   "ENU mutagenesis screening for dominant behavioral mutations based on normal control data obtained in Home-cage activity, open-field, and passive avoidance tests"
   Experimental Animals, 59(4):495-510 (2010)
7. Furuse T., Wada Y., Hattori K., Yamada I., Kushida T., Shibukawa Y., Masuya H., Kaneda H., Miura I., Seno N., Kanda T., Hirose R., Toki S., Nakanishi K., Kobayashi K., Sezutsu H., Gondo Y., Noda T., Yuasa S., Wakana S.:
   "Phenotypic characterization of a new Grin1 mutant mouse generated by ENU mutagenesis"
   European Journal of Neuroscience, 31:1281-1291 (2010)
8. Sato H., Suzuki T., Ikeda K., Masuya H., Sezutsu H., Kaneda H., Kobayashi K., Miura I., Kurihara Y., Yokokura S., Nishida K., Tamai M., Gondo Y., Noda T., Wakana S.:
   "A monogenic dominant mutation in Rom1 generated by N-ethyl-N-nitrosourea mutagenesis causes retinal degeneration in mice"
   Molecular Vision, 16:378-391 (2010)
9. Wakana S, Suzuki T, Furuse T, Kobayashi K, Miura I, Kaneda H, Yamada I, Motegi H, Toki H, Inoue M, Minowa O, Noda T, Waki K, Tanaka N, Masuya H, Obata Y.:
   "Introduction to the Japan Mouse Clinic at the RIKEN BioResource Center."
   Exp Anim. 58(5):443-450 (2009)
10. Taguma K, Nakamura C, Ozaki A, Suzuki C, Hachisu A, Kobayashi K, Mochida K, Ogura A, Kaneda H, Wakana:
    "A practical novel method for ensuring stable capacitation of spermatozoa from cryopreserved C57BL/6J sperm suspension."
    Exp Anim. 58(4):395-401 (2009)