Technology and Development Team for Mammalian Genome Dynamics

Aim of our team is to develop technologies and experimental tools/ resources for characterization of 'genotype, phenotype and epigenotype' of biological resources. Through these efforts, we would extend utilities of bioresources collected at RIKEN BRC. Based on these technologies and resources, we will establish analytical platform for analyses of dynamic nature of mammalian genome in response to changes in various environmental factors. Using this platform, we will be able to explore how environmental factors influence on development and growth of organisms, or on the onset of disease condition.

Research Fields

Biology & Biochemistry / Molecular Biology & Genetics / Plant & Animal Science / Multidisciplinary

Research Subjects

• Technology development for comprehensive analysis of epigenome of small number of cells
• Technology development for visualization and imaging analyses of biological processes
• Identification and functional characterizations of genes related to mammalian development and diseases using mutant resources

Publications

1. Sugimoto, M., Kondo, M., Hirose, M., Suzuki, M., Mekada, K., Abe, T., Kiyonari, H., Ogura, A., Takagi, N., Artzt, K. and Abe, K.
   "Molecular identification of t^w5: Vps52 promotes pluripotential cell differentiation through cell-cell interactions."
   Cell Reports 2, 1363-1374. (2012)
2. Okamura, D., Maeda, I., Taniguchi, H., Tokitake, Y., Ikeda, M., Ozato, K., Mise, N., Abe, K., Noce, T., Belmonte, J.C.I., Matsui, Y.
   "Cell-cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells."
   Genes Dev. 26, 2477-2482. (2012)
   
3. Cao, L, Kobayakawa, S., Yoshiki, A., Abe, K.
   "High resolution intravital imaging of subcellular structures of mouse abdominal organs using a microstage device."
   PLoS One e33876. (2012)
   
4. Inoue, K., Kohda, T., Sugimoto, M., Sado, T., Ogonuki, N., Matoba, S., Shiura, H., Ikeda, R., Mochida, K., Fujii, T., Sawai, K., Otte, A.P., Tian, X.C., Yang, X., Ishino, F., Abe, K. and Ogura, A.
   "Impeding Xist expression from the active X chromosome improves mouse somatic cell cloning."
   Science 330, 496-499. (2010)
5. Kasahara, T., Abe, K., Mekada, K., Yoshiki, A., Kato, T.
   "Genetic variation of melatonin productivity in laboratory mice under domestication."
   Proc. Natl. Acad. Sci, USA 107, 6412-6417. (2010)
   
6. Cao, L., Shitara, H., Sugimoto, M., Hayashi, J.=I., Abe, K., Yonekawa, H.
   "New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice."
   PLoS Genetics 5, e1000756. (2009)
   
7. Tomida S, Mamiya T, Sakamaki H, Miura M, Aosaki T,Masuda M, Niwa M, Kameyama T, Kobayashi J, Iwaki Y, Imai S, Ishikawa A, Abe K, Yoshimura T, Nabeshima T & Ebihara S.
   "Usp46, encoding a ubiquitin specific peptidase, is a quantitative trait gene regulating mouse immobile behavior in the tail suspension and forced swimming tests."
   Nature Genetics 41, 688-695. (2009)
   
8. Mise, N., Fuchikami, T., Sugimoto, M., Kobayakawa, S., Ike, F., Ogawa, T., Tada, T., Kanaya, S., Noce, T. and Abe, K.
   "Differences and similarities in the developmental status of embryo-derived stem cells and primordial germ cells revealed by global expression profiling."
   Genes cells 13, 863-877. (2008)
   
9. Sugimoto, M. and Abe, K.
   "X chromosome reactivation initiates in nascent primordial germ cells in mice."
   PLoS Genetics 3, e116. (2007)
   
10. Kobayakawa, S., Miike, K., Nakao, M. and Abe, K.
    "Dynamic changes in the epigenomic state and nuclear organization of differentiating mouse embryonic stem cells."
    Genes Cells 12, 447-460. (2007)