Cell Engineering Division (RIKEN BRC Cell Bank)

"Cell Engineering Division in RIKEN BioResource Center (BRC)" was originally organized as the "RIKEN Cell Bank" in 1987. It is a unique, not-for-profit public organization for deposit, standardization, preservation, and distribution of cultured animal cell lines produced by life science community. We conduct not only banking of cell materials but also research and development related to cell engineering, such as establishment of novel human and animal cell lines and development of technology to culture primate pluripotent stem cells efficiently.

Research Subjects

• Development of fundamental technologies relating to ES and iPS cells
• Development of technologies to induce differentiation from ES or iPS cells
• Development of technologies to establish cell lines possessing differentiation potential

Publications

1. Masters, J. R., Alston-Roberts, C., Barrett, T., Burnett, E. C., Cooper, J. R., Dirks, W. G., Freshney, R. I., Healy, L., Kerrigan, L., Kohara, A., Korch, C., MacLeod, R. A. F., Nakamura, Y., Nims, R. W., Reid, Y. A., Storts, D. R., and Capes-Davis, A.:
   "End the scandal of false cell lines."
   Nature 492: 186 (2012)
2. Danjoh, I., Shirota, R, Hiroyama, T., and Nakamura, Y.:
   "Dominant expansion of a cryptic subclone with an abnormal karyotype in B lymphoblastoid cell lines during culture. "
   Cytogen. Genome Res. 139: 88-96 (2013)
3. Danjoh, I., Sone, H., Shirota, R., Hiroyama, T., and Nakamura, Y.:
   "Development of a robust method for establishing B cell lines using Epstein-Barr Virus."
   In Vitro Cell. Dev. Biol. - Animal 48: 393-402 (2012)
4. Danjoh, I., Saijo, K., Hiroyama, T., and Nakamura, Y.:
   "The Sonoda-Tajima Cell Collection, a human genetics research resource with emphasis on South American indigenous populations."
   Genome Biol. Evol. 3: 272-283, open access journal, doi:10.1093/gbe/evr014 (2011)
5. Sudo, K., Yasuda, J., and Nakamura, Y.:
   "Gene expression profiles of cryopreserved CD34+ human umbilical cord blood cells are related to their bone marrow reconstitution abilities in mouse xenografts."
   Biochem. Biophys. Res. Commun. 397: 697-705 (2010)
6. Ishigaki, T., Sudo, K., Hiroyama, T., Miharada, K., Ninomiya, H., Chiba, S., Nagasawa, T., and Nakamura, Y.:
   "Human hematopoietic stem cells can survive in vitro for several months."
   Adv. Hematol. 2009: ID936761 (open access journal) (2009)
7. Danjoh, I., Sone, H., Sekiyama, S., Mizukoshi, K., Noda, N., Iimura, E., Nagayoshi, M., Saijo, K., Hiroyama, T., and Nakamura, Y.:
   "Is parainfluenza virus a threatening virus for human cancer cell lines?"
   Hum. Cell 22: 81-84 (2009)
8. Hiroyama, T., Miharada, K., Sudo, K., Danjo, I., Aoki, N., and Nakamura, Y.:
   "Establishment of mouse embryonic stem cell-derived erythroid progenitor cell lines able to produce functional red blood cells."
   PLoS ONE 3: e1544 (2008).
9. Sudo, K., Kanno, M., Miharada, K., Ogawa, S., Hiroyama, T., Saijo, K., and Nakamura, Y.:
   "Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations."
   Stem Cells 25: 1610-1617 (2007)
10. Miharada, K., Hiroyama, T., Sudo, K., Nagasawa, T., and Nakamura, Y.:
    "Efficient enucleation of erythroblasts differentiated in vitro from hematopoietic stem and progenitor cells."
    Nat. Biotechnol. 24: 1255-1256 (2006)

Organization