Technology and Development Team for BioSignal Program

• Regulatory mechanisms of immune system including autoimmune and inflammation respons by NF-kB/RelA.
• NF-kB/RelA regulation of bone metabolism.
• Regulatory mechanisms of hematopoiesis systems by NF-kB/RelA.
• Development of methods to expand hematopoietic stem cell in vitro.
• Development of methods for efficient generation and stable expansion of pluripotent stem cells (ES and iPS cells).
• Development of viral vectors to transfer genes into cells and their use in stem cell research.

Research Fields

Biology & Biochemistry / Molecular Biology & Genetics / Immunology / Clinical Medicine / Plant & Animal Science

Research Subjects

• Regulatory mechanism of hematopoietic stem cells
• Control mechanism of immune system by NF-kB/RelA
• Development of lentiviral vectors and their application to stem cell biology

Publications

1. Nakano H, Doi T et al.
   Oxidative stress-dependent production of Interleukin 11 ameliorates drug-induced murine hepatitis through compensatory proliferation.
   Journal of Clinical Investigation (2011)
2. Maruyama T, Fukushima H, Nakao K, Shin M, Yasudsa H, Weih F, Doi T, Aoki K, Ohya K, Hsokawa R and Jimi E
   Processing of the NF-kB2 precursor, p100, to p52 is critical for RANKL-induced osteoclast differentiation
   Journal of Bone and Mineral Research (2009)
3. Yamazaki M, Fukushima H, Shin M, Katagiri T, Doi T, Takahashi T and Jimi E
   A proximal kappaB site in the IL-23 p19 promoter is responsible for RelA- and c-Rel-dependent transcription
   Journal of Biological Chemistry, 284; 35987-95 (2009)
4. Mise-Omata S, Kuroda E, Sugiura T, Yamashita U, Obata Y, and Doi TS
   The NF-kB RelA subunit confers resistance to Leishmania major by inducing NOS2 and Fas expression but not Th1 differentiation
   J. Immunol. 182;4910-4916 (2009)
5. Mise-Omata S, Kuroda E, Niikura J, Yamashita U, Obata Y, and Doi TS
   A proximal kappaB site in the IL-23 p19 promoter is responsible for RelA- and c-Rel-dependent transcription
   J. Immunol. 179, 6596-6603 (2007)
6. Inoue, S., Noda, S., Kashima, K., Nakada, K., Hayashi, J., and Miyoshi, H.
   Mitochondrial respiration defects modulate differentiation but not proliferation of hematopoietic stem and progenitor cells.
   FEBS Lett. 584, 3402-3409 (2010)
7. Noda, S., Ichikawa, H., and Miyoshi, H.
   Hematopoietic stem cell aging is associated with functional decline and delayed cell cycle progression.
   Biochem. Biophys. Res. Commun. 383, 210-215 (2009)
8. Shimizu, N., Noda, S., Katayama, K., Ichikawa, H., Kodama, H., and Miyoshi, H.
   Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6.
   Int. J. Hematol. 87, 239-245 (2008)
9. Noda, S., Horiguchi, K., Ichikawa, H., and Miyoshi, H.
   Repopulating activity of ex vivo-expanded murine hematopoietic stem cells resides in the CD48-c-Kit+Sca-1+lineage marker- cell population.
   Stem Cells 26, 646-655 (2008)
10. Inoue, S., Yokota, M., Nakada, K., Miyoshi, H., and Hayashi, J.
    Pathogenic mitochondrial DNA-induced respiration defects in hematopoietic cells result in anemia by suppressing erythroid differentiation.
    FEBS Lett. 581, 1910-1916 (2007)