Bioresource Engineering Division

To support the projects undertaken by the Bioresource Project, we develop new techniques for practical genetics, which are essential for consistent, high quality maintenance and supplies of bioresources. The techniques which are to be developed immediately include those related to nuclear transfer, establishment of stem cell lines of various origins, and cryopreservation of embryos/gametes.

Research Subjects

• Establishment of methods for cryopreservation of embryos and germ cells
• Establishment of methods for microinsemination (ICSI)
• Establishment of methods for nuclear transfer cloning
• Establishment of new stem cell lines for research use

Publications

1. Matoba S, Inoue K, Kohda K, Sugimioto M, Mizutani E, Ogonuki N, Nakamura T, Abe K, Nakano T, Ishino F, and Ogura A.:
   "RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos. "
   Proc Natl Acad Sci USA, 108, 20621-20626 (2011)
2. Inoue K, Kohda T, Sugimoto M, Sado T, Ogonuki N, Matoba S, Shiura H, Ikeda R, Mochida K, Fujii T, Sawai K, Otte AP, Tian XC, Yang X, Ishino F, Abe K, Ogura A.:
   "Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer."
   Science, 330, 496-499 (2010)
3. Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A.:
   "Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells."
   Proc Natl Acad Sci USA, 104, 12389-12394 (2007)
4. Ogonuki, N., Mochida, K., Miki, H., Inoue, K., Fray, M., Iwaki, T., Moriwaki, K., Obata, Y., Morozumi, K., Yanagimachi, R., and Ogura A.:
   "Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring"
   Proc. Natl. Acad. Sci. USA, 103, 13098-13103 (2006)
5. Inoue, K., Ogonuki, N., Miki, H., Hirose, M., Noda, S., Kim, J-M., Aoki, F., Miyoshi, H,, and Ogura A.:
   "Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer"
   J. Cell Sci. 119, 1985-1991(2006)
6. Inoue, K., Wakao, H., Ogonuki, N., Miki, H., Seino, K., Nambu-Wakao, R., Noda, S., Miyoshi, H., Koseki, H., Taniguchi, M., and Ogura, A.:
   "Generation of cloned mice by direct nuclear transfer from natural killer T cells"
   Curr. Biol. 15, 1114-1118 (2005)
7. Kanatsu-Shinohara, M., Inoue, K., Lee, J., Yoshimoto, M., Ogonuki, N., Miki, H., Baba, S., Kato, T., Kazuki, Y., Toyokuni, S., Oshimura, M., Heike, T., Nakahata, T., Ishino, F., Ogura, A., and Shinohara, T.:
   "Generation of pluripotent stem cells from neonatal mouse testis"
   Cell 119, 1001-1012 (2004)
8. Fulka, J Jr., Miyashita, N., Nagai, T., and Ogura, A.:
   "Do cloned mammals skip a reprogramming step"
   Nat. Biotechnol. 22, 25-26 (2004)
9. Ogonuki, N., Inoue, K., Yamamoto, Y., Noguchi, Y., Tanemura, K., Suzuki, O., Nakayama, H., Doi, K., Ohtomo, K., Satoh, M., Nishida, A., and Ogura, A.:
   "Early death of mice cloned from somatic cells"
   Nat. Genet. 30, 253-254 (2002)
10. Inoue, K., Kohda, T., Lee, J., Ogonuki, N., Mochida, K., Noguchi, Y., Tanemura, K., Kaneko-Ishino, T., Ishino, F., and Ogura, A.:
    "Faithful expression of imprinted genes in cloned mice"
    Science 295, 297 (2002)